Multisignal labeling reagents, and processes and uses therefor
The present invention provides multisignal labeling reagents and these are useful in a number of biochemical applications, including the manufacture of biomolecular probes and their use in detecting or amplifying analyte-specific moieties.
1. A composition of matter comprising a nucleic acid strand or a complex of two or more nucleic acid strand wherein said strand or complex comprises one or more reactive groups and two or more labeled nucleotides or labeled nucleotide analogs, wherein said reactive group is isothiocyanate, isocyanate, monochlorotriazine, dichlorotriazine, mono- or di-halogen substituted pyridine, mono- or di-halogen substituted diazine, maleimide, aziridine, sulfonyl halogen substituted diazine, maleimide, aziridine, sulfonyl halide, acid halide, hydroxysuccinimide ester, hydroxysulfosuccinimide ester, imido ester, hydrazine, azidonitrophenyl, azide, 3-(2-pyridyl dithio)-proprionamide, glyoxal, aldehyde, carbon-carbon double bonds, mercury salts, and/or any group capable of reacting with carbon-carbon double bonds, amines, hydroxyl groups, sulfhydryl groups and/or halogens.
2. The composition of claim 1 , wherein said nucleic acid strand or complex comprise one or more unlabeled nucleotide analogs.
3. The composition of claims 1 or 2 , wherein said nucleotide analogs are a basic nucleotides, spacer groups or peptide nucleic acid subunits.
4. The composition of claim 1 , wherein said label is a fluorescent compounds, phosphorescent compounds, chemiluminescent compounds, chelating compounds, electron dense compounds, magnetic compounds, intercalating compounds and/or energy transfer compounds or any combination thereof.
5. The composition of claim 1 , wherein said reactive group is attached to said strand or complex through a linker.
6. The composition of claim 5 , wherein said linker is attached to a nucleotide or nucleotide analog comprising said strand or complex and said attachment is through a base, sugar, phosphate or an analog thereof.
7. The composition of claim 5 , wherein said linker comprises one or more carbon, nitrogen, oxygen, phosphorus or sulfur atoms in any combination.
8. The composition of claim 5 , wherein said linker comprises one or more peptide bonds, alkyl chains, alkene groups, alkyl groups, aryl groups, conjugated systems, sugars or derivatives thereof.
9. The composition of claim 1 , wherein said nucleic acid strands are linear or branched.
10. The composition of claim 4 , wherein said complex is formed through base-pairing or triple strand formation.