Cells in which activity of the protein involved in transportation of GDP-fucose is reduced or lost
View Patent ↗A cell in which the activity of a protein relating to transport of an intracellular sugar nucleotide, GDP-fucose, to the Golgi body is more decreased or deleted than its parent cell; a process for producing an antibody composition using the cell; a transgenic non-human animal or plant or the progenies thereof, in which genome is modified so as to have a decreased or deleted activity of a protein relating to transport of an intracellular sugar nucleotide, GDP-fucose, to the Golgi body; a process for producing an antibody composition from the animal or plant; and a medicament comprising the antibody composition.
1. An isolated cell in which the activity of a GDP-fucose transporter is deleted by a gene disruption technique which comprises targeting a gene encoding a GDP-fucose transporter, wherein the gene encoding the GDP-fucose transporter is a DNA comprising the nucleotide sequence of SEQ ID NO:1.
2. An isolated cell in which the activity of a GDP-fucose transporter is deleted by a gene disruption technique which comprises targeting a gene encoding a GDP-fucose transporter, wherein the GDP-fucose transporter is a protein comprising the amino acid sequence of SEQ ID NO:2.
3. The cell according to claim 1 or claim 2 , which is resistant to a lectin which recognizes a sugar chain in which 1-position of fucose is bound to 6-position of N-acetylglucosamine in the reducing end through α-bond in a complex N-glycoside-linked sugar chain.
4. The cell according to claim 3 , which is resistant to at least one lectin selected from the group consisting of the following (a) to (d):
(a) a Lens culinaris lectin;
(b) a Pisum sativum lectin;
(c) a Vicia faba lectin;
(d) an Aleuria aurantia lectin.
5. The cell according to claim 1 or claim 2 , wherein the cell is an animal cell.
6. An isolated transformed cell in which the activity of a genomic GDP-fucose transporter is deleted, wherein the genomic GDP-fucose transporter is a protein comprising the amino acid sequence of SEQ ID NO:2; and wherein the activity of the genomic GDP-fucose transporter is deleted by an RNA interference (RNAi) method.
7. The cell according to claim 6 , wherein the RNAi method uses a nucleotide represented by SEQ ID NO: 16.
8. The cell according to claim 6 , which is resistant to a lectin which recognizes a sugar chain in which 1-position of fucose is bound to 6-position of N-acetylglucosamine in the reducing end through α-bond in a complex N-glycoside-linked sugar chain.
9. The cell according to claim 8 , which is resistant to at least one lectin selected from the group consisting of the following (a) to (d):
(a) a Lens culinaris lectin;
(b) a Pisum sativum lectin;
(c) a Vicia faba lectin;
(d) an Aleuria aurantia lectin.
10. The cell according to claim 6 , wherein the cell is an animal cell.
11. The cell according to claim 10 , wherein the animal cell is a CHO cell.