Nucleic acid marker ladder for estimating mass
View Patent ↗The invention relates to a nucleic acid marker ladder which is a restriction endonuclease digest, wherein a nucleic acid restriction endonuclease digest is a collection of nucleic acid fragments resulting from complete digestion of one or more nucleic acids by one or more restriction endonucleases; the restriction endonuclease digest contains at least 3 fragments; and the size of the fragments in base pairs is a multiple of an integer, wherein the integer is 10 or more.
1. A nucleic acid marker ladder consisting essentially of 3 to 10 nucleic acid fragments, wherein the size of each said fragment in base pairs is a multiple of an integer between 10 and 100, and wherein the mass of each said fragment is equal to its size in base pairs divided by sum of the sizes in base pairs of all said fragments times the total mass of all said fragments.
2. The nucleic acid marker ladder of claim 1 , wherein said fragments are derived from one or more nucleic acid molecules by digestion with one or more restriction endonucleases.
3. The nucleic acid marker ladder of claim 2 , wherein said fragments are derived from a single nucleic acid molecule.
4. The nucleic acid marker ladder according to claim 1 , wherein the integer is 10.
5. The nucleic acid marker ladder according to claim 1 , wherein the integer is 25.
6. The nucleic acid marker ladder according to claim 1 , wherein the integer is 50.