Multi channel capillary electrophoresis device and method
View Patent ↗Embodiments of a device and method are described which provide for control over the distortion of a sample zone upon exiting an electrophoresis separation channel. According to the teachings herein (i) the downstream regions of the channels, near the outlet ends, and/or (ii) the detection chamber, is/are configured so that distortion of one or more sample zones passing from the channels into and across the detection chamber can be controlled (e.g., reduced) in a fashion affording enhanced detectability. In certain embodiments, the lumens along the end regions of the separation channels progressively expand.
1. A device for analyzing one or more samples, comprising:
a plurality of channels, each channel comprising an inlet end and an outlet end; wherein each of said channels defines, at least in part, a sample-migration pathway that extends longitudinally along at least a portion of such channel;
a detection zone downstream of said inlet ends; wherein each of said sample-migration pathways traverses at least a portion of said detection zone;
an excitation-beam pathway that extends through at least a portion of said detection zone and intersects each of said sample-migration pathways;
a detector disposed to simultaneously detect radiation emitted from a plurality of spaced-apart observation regions within said detection zone, each of said observation regions being located whereat said excitation-beam pathway intersects a respective one of said sample-migration pathways; and
a plurality of crosstalk zones, each crosstalk zone being located between a respective pair of adjacent observation regions;
wherein said detector is configured to disregard radiation emitted from said crosstalk zones.
2. The device of claim 1 , wherein said radiation comprises fluorescence.
3. The device of claim 1 , wherein each of said sample-migration pathways passes through a respective outlet end.
4. The device of claim 1 , wherein said detector comprises a CCD camera.
5. The device of claim 1 , further comprising a separation medium supported by said channels.
6. A device for analyzing one or more samples, comprising:
a plurality of channels; wherein each of said channels defines, at least in part, a sample-migration pathway that extends longitudinally along at least a portion of such channel;
a detection zone; wherein each of said sample-migration pathways traverses at least a portion of said detection zone;
an excitation-beam pathway that intersects said sample-migration pathways in said detection zone;
a detector disposed to detect radiation emitted from a plurality of spaced-apart observation regions within said detection zone, each of said observation regions being located whereat said excitation-beam pathway intersects a respective one of said sample-migration pathways; and
a plurality of crosstalk zones, each crosstalk zone being located between a respective pair of adjacent observation regions;
wherein said detector is configured to disregard radiation emitted from said crosstalk zones.
7. The device of claim 6 , wherein said radiation comprises fluorescence.
8. The device of claim 6 , wherein said detector comprises a CCD camera.
9. The device of claim 6 , further comprising a separation medium supported by said channels.
10. A device for analyzing one or more samples, comprising:
a plurality of channels; wherein each of said channels defines, at least in part, a sample-migration pathway that extends longitudinally along at least a portion of such channel;
an excitation-beam pathway that intersects each of said sample-migration pathways;
a detector disposed to simultaneously detect radiation emitted from a plurality of spaced-apart observation regions, each of said observation regions being located whereat said excitation-beam pathway intersects a respective one of said sample-migration pathways; and
a plurality of crosstalk zones, each crosstalk zone being located between a respective pair of adjacent observation regions;
wherein said detector is configured to disregard radiation emitted from said crosstalk zones.
11. The device of claim 10 , wherein said radiation comprises fluorescence.
12. The device of claim 10 , wherein said detector comprises a CCD camera.
13. The device of claim 10 , further comprising a separation medium supported by said channels.
14. A method for analyzing a plurality of sample zones migrating along respective sample-migration pathways, comprising:
(a) directing an excitation beam along a beam pathway which extends along a plane defined by said sample-migration pathways;
(b) simultaneously detecting for radiation emitted from a plurality of spaced-apart observation regions, each of said observation regions being located whereat said excitation-beam pathway intersects one of said sample-migration pathways; and
(c) disregarding radiation emitted from any one or more of a plurality of crosstalk zones, wherein each crosstalk zone is located midway between a respective pair of adjacent observation regions.
15. A method for reducing distortion of sample zones upon exiting separation channels, comprising:
(a) loading a plurality of separation channels with respective samples;
(b) applying a motive force along the channels thereby causing the samples to migrate through the channels and resolve into one or more sample zones; and
(c) transferring each sample zone from its respective channel into a detection zone having a cross-sectional area, taken along a plane perpendicular to the direction of sample zone travel, within a range of from 80% to 150% of the sum of the cross-sectional areas of all of the channels, wherein the channel cross-sectional area employed in calculating such sum is the cross-sectional area predominating along most of the channel length.
16. The method of claim 15 , wherein said detection chamber cross-sectional area is within a range of from 90% to 110% of the sum of the cross-sectional areas of all of the channels.
17. A method for reducing distortion of a sample zone upon exiting an electrophoretic channel containing a separation medium, comprising:
(a) loading a sample at an inlet end of said channel;
(b) applying an electric field along the channel thereby causing the sample to migrate through the medium and resolve into one or more sample zones;
(c) changing the cross-sectional area of each sample zone as the zone passes along the vicinity of an outlet end of said channel, said cross-sectional area taken along a plane perpendicular to the direction of sample zone travel;
(d) ejecting the sample zone from the channel via the outlet end; and
(e) analyzing the sample zone for the presence of one or more analytes of interest, said analyzing comprising (i) directing an excitation beam along a beam pathway which is located downstream of said channels and which extends along a plane defined by said channels; and (ii) simultaneously detecting for fluorescence emitted from a plurality of spaced-apart observation regions, each of said observation regions being located whereat said excitation-beam pathway intersects an axial extension of a respective one of said channels.
18. A method for reducing distortion of sample zones upon exiting electrophoretic channels containing a separation medium, comprising:
(a) loading a plurality of separation channels with respective samples;
(b) applying an electric field along the channels thereby causing the samples to migrate through the medium and resolve into one or more sample zones;
(c) transferring each sample zone from its respective channel into a detection zone having a cross-sectional area, taken along a plane perpendicular to the direction of sample zone travel, within a range of from 50% to 400% of the sum of the cross-sectional areas of all of the channels;
(d) directing an excitation beam along a beam pathway that traverses at least a portion of said detection chamber, downstream of said channels, and extends along a plane defined by said channels; and
(e) simultaneously detecting for fluorescence emitted from a plurality of spaced-apart observation regions, each of said observation regions being located whereat said excitation-beam pathway intersects an axial extension of a respective one of said channels.