Tests for the rapid evaluation of ischemic states and kits
View Patent ↗The present invention relates to rapid methods for the detection of ischemic states and to kits for use in such methods. Provided for is a rapid method of testing for and quantifying ischemia based upon methods of detecting and quantifying the existence of an alteration of the serum protein albumin which occurs following an ischemic event; methods for detecting and quantifying this alteration include evaluating and quantifying the cobalt binding capacity of circulating albumin, analysis and measurement of the ability of serum albumin to bind exogenous cobalt, detection and measurement of the presence of endogenous copper in a purified albumin sample and use of an immunological assay specific to the altered form of serum albumin which occurs following an ischemic event. Also taught by the present invention is the detection and measurement of an ischemic event by measuring albumin N-terminal derivatives that arise following an ischemic event, including truncated albumin species lacking one to four N-terminal amino acids or albumin with an acetylated N-terminal Asp residue.
1. A method of detecting an albumin derivative N-terminus epitope, wherein said albumin derivative N-terminus epitope comprises the 4-12 amino acids at the N-terminus of albumin after removal of 4, 3, 2 or 1 N-terminal residues or the 4-12 amino acids at the N-terminus of albumin that has been acetylated at its N-terminal Asp residue, said method comprising:
contacting a blood, serum or plasma sample comprising said derivative with an antibody that specifically binds to the albumin derivative N-terminus epitope, and
detecting antibody which has bound to said albumin derivative N-terminus epitope.
2. The method of claim 1 , wherein the antibody is specific to the albumin derivative N-terminus epitope which lacks four N-terminal amino acids of SEQ. ID. NO. 1.
3. The method of claim 1 , wherein the antibody is specific to the albumin derivative N-terminus epitope which lacks three N-terminal amino acids of SEQ. ID. NO. 1.
4. The method of claim 1 , wherein the antibody is specific to the albumin derivative N-terminus epitope which lacks two N-terminal amino acids of SEQ. ID. NO. 1.
5. The method of claim 1 , wherein the antibody is specific to the albumin derivative N-terminus epitope which lacks an N-terminal amino acid of SEQ. ID. NO. 1.
6. The method of claim 1 , wherein the antibody is specific to the albumin derivative N-terminus epitope which is acetylated at its N-terminal Asp residue (SEQ. ID. NO. 2).