Process for producing D-serine
View Patent ↗The present invention relates to: a process for producing D-serine wherein a microbial cell which is modified to have a higher L-serine deaminase activity than Escherichia coli DH5α strain, a culture of said cell, or a processed product thereof is brought into contact with DL-serine in a DL-serine-containing medium to decompose L-serine, and the remaining D-serine is recovered from the medium; and a microorganism used for this production process. D-serine is a useful compound as a synthetic intermediate for useful medicaments such as D-cycloserine.
1. A process for producing D-serine, which comprises bringing a microorganism, or a processed product thereof comprising the E. coli sdaA or adaB gene product into contact with DL-serine in a DL-serine-containing medium to decompose L-serine, and recovering remaining D-serine from the medium, wherein said microorganism is obtained by a method comprising,
introducing an Escherichia coli sdaA or sdaB gene into a microorganism to obtain a transformed microorganism,
measuring the L-serine deaminase activity of the transformed microorganism, and
selecting a transformed microorganism wherein the transformed microorganism has a higher L-serine deaminase activity than Escherichia coli DH5α strain.
2. The process for producing D-serine according to claim 1 , wherein the L-serine deaminase activity of the transformed microorganism is twice or higher compared to that of Escherichia coli DH5α strain.
3. The process for producing D-serine according to claim 1 , wherein the L-serine deaminase activity of the transformed microorganism is five times or higher compared to that of Escherichia coli DH5α strain.
4. The process for producing D-serine according to claim 1 , wherein the transformed microorganism into a microorganism is a microorganism belonging to Escherichia coli.
5. The process for producing D-serine according to claim 1 , wherein the microorganism is selected from the group consisting of Esoharichia coli DH5α/pHIB, Escherichia coli NM522/pHIA1 , Escherichia coli MM294/pHIA1 , Escherichia coli MM294/pHIA2 , Escherichia coli MM294/pH 18, and Escherichia coli ME53866/pHIA2.
6. The process for producing D-serine according to claim 1 , wherein the microorganism has low or no D-serine deaminase activity.
7. An Escherichia coli microorganism which is obtained by introducing an Escherichia coli sdaA or sdaB gene into the microorganism, which is selected from the group consisting of Escherichia coli DH5α/pHIB, Escherichia coli NM522/pHIA1 , Escherichia coli MM294/pHIA1 , Escherichia coli MM294/pHIA2 , Escherichia coli MM294/pHIB, and Escherichia coli ME5386/pHIA2.