IP Library › Granted Patent US 7,011,966
Granted Patent B2
US 7,011,966 · App. 10/417,293 · Granted Mar 14, 2006

Method for cloning and expression of AcuI restriction endonuclease and AcuI methylase in E. coli

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Quick Facts
Patent No.
US 7,011,966
App. No.
10/417,293
Granted
Mar 14, 2006
Kind
B2
Abstract

The present invention relates to recombinant DNA encoding the AcuI restriction endonuclease as well as AcuI methylase, and expression of AcuI restriction endonuclease and AcuI methylase in E. coli cells containing the recombinant DNA.

Claims (6)

1. Isolated DNA encoding the AcuI restriction endonuclease, wherein the isolated DNA is obtainable from Acinetobacter calcoaceticus SRW4.

2. A recombinant DNA vector comprising a vector into which a DNA segment encoding the AcuI restriction endonuclease has been inserted.

3. Isolated DNA encoding the AcuI restriction endonuclease and AcuI methylase, wherein the isolated DNA is obtainable from ATCC No. PTA-1513.

4. A cloning vector that comprise the isolated DNA of claim 3 .

5. A host cell transformed by the vector of claim 2 or 4 .

6. A method of producing recombinant AcuI restriction endonuclease comprising culturing the host cell of claim 5 under conditions suitable for expression of said endonuclease and methylase.

Assignments (1)
TERMINATION AND RELEASE OF SECURITY INTEREST IN PATENTS Recorded Sep 20, 2023
From: BANK OF AMERICA, N.A. (AS SUCCESSOR-BY-MERGER WITH FLEET NATIONAL BANK)
To: NEW ENGLAND BIOLABS, INC.
Reel/Frame 064962/0066 →