Method for obtaining the contents of a fluid-holding vessel
View Patent ↗A method for obtaining the contents of a fluid-holding vessel having a cap secured to an open end thereof. The method includes penetrating a surface of the cap with a fluid transfer device and forming one or more air passageways between the penetrated surface of the cap and the fluid transfer device to allow air to be vented from the vessel. At least a portion of the contents of the vessel is then drawn into the fluid transfer device before the fluid transfer device is removed from the vessel.
1. A method for obtaining the contents of a closed, fluid-holding vessel, the method comprising the steps of:
penetrating a top surface of a single-piece plastic cap secured to an open end of a fluid-holding vessel with a plastic pipette tip of a fluid transfer device, thereby forming one or more passageways between the top surface of the cap and the fluid transfer device for venting air from within the vessel;
drawing at least a portion of the contents of the vessel into the fluid transfer device; and
removing the fluid transfer device from the vessel.
2. The method of claim 1 , wherein the contents of the vessel include a biological fluid selected from the group consisting of blood, urine, saliva, sputum, mucous or other bodily secretion, pus, amniotic fluid, cerebrospinal fluid and seminal fluid.
3. The method of claim 1 , wherein the vessel contains a specimen retrieval device having a handle member spaced from the longitudinal axis of the vessel and positioned along an inner surface of a side wall of the vessel.
4. The method of claim 1 , further comprising providing a lubricant to an outer surface of the pipette tip or to the top surface of the cap prior to the penetrating step.
5. The method of claim 1 , wherein the cap contains a wick positioned above the top surface for limiting the release of an aerosol from the vessel during the penetrating step.
6. The method of claim 5 , wherein the wick is selected from the group consisting of a fabric, a sponge and a foam.
7. The method of claim 6 , wherein the wick is a pile fabric.
8. The method of claim 5 further comprising the step of performing an amplification procedure with at least a portion of the contents contained in the fluid transfer device during the removing step to detect the presence of a target nucleic acid suspected of being present in the contents.
9. The method of claim 8 , wherein the amplification procedure is a form of the polymerase chain reaction or a transcription-based amplification.
10. The method of claim 5 further comprising penetrating a seal affixed to the cap with the pipette tip, wherein the seal maintains the wick within the cap.
11. The method of claim 1 , wherein the pipette tip includes one or more integrally formed, solid ribs extending outwardly from an outer surface thereof, and wherein at least one of the ribs contacts the top surface of the cap during the penetrating step.
12. The method of claim 11 , wherein the pipette tip includes a beveled tip.
13. The method of claim 1 , wherein the pipette tip includes one or more grooves recessed from an outer surface thereof, and wherein at least one of the passageways is formed between at least one of the grooves and the top surface of the cap during the penetrating step.
14. The method of claim 13 , wherein the pipette tip further includes one or more integrally formed, solid ribs extending outwardly from an outer surface thereof, and wherein at least one of the ribs contacts the top surface of the cap during the penetrating step.
15. The method of claim 14 , wherein at least one of the grooves overlaps at least one of the ribs on the outer surface of the pipette tip.
16. The method of claim 1 , wherein the top surface of the cap includes one or more integrally formed, radially extending ribs, wherein at least one of the ribs contacts the pipette tip during the penetrating step.
17. The method of claim 16 , wherein the top surface of the cap is an inner surface of a generally conical inner wall.
18. The method of claim 17 , wherein the inner wall includes a plurality of radially extending striations.
19. A method for obtaining the contents of a closed, fluid-holding vessel, the method comprising the steps of:
penetratin a top surface of a cap secured to an open-end of a fluid-holding vessel with a fluid transfer device having one or more solid ribs extending outwardly from an outer surface thereof, thereby forming one or more passagewavs between the top surface of the cap and the fluid transfer device for venting air from within the vessel;
drawing at least a portion of the contents of the vessel into the fluid transfer device; and
removing the fluid transfer device from the vessel.
20. The method of claim 19 , wherein the vessel contains a specimen retrieval device having a handle member spaced from the longitudinal axis of the vessel and positioned along an inner surface of a side wall of the vessel.
21. The method of claim 19 , wherein the cap is one piece plastic cap, and wherein the top surface of the cap is an inner surface of a generally conical inner wall.
22. The method of claim 21 , wherein the inner wall includes a plurality of radially extending striations.
23. The method of claim 19 , wherein the cap contains a wick positioned above the top surface for limiting the release of an aerosol from the vessel during the penetrating step.
24. The method of claim 23 , wherein the wick is selected from the group consisting of a fabric, a sponge and a foam.
25. The method of claim 24 , wherein the wick is a pile fabric.
26. The method of claim 23 further comprising the step of performing an amplification procedure with at least a portion of the contents contained in the fluid transfer device during the removing step to detect the presence of a target nucleic acid suspected of being present in the contents.
27. The method of claim 26 , wherein the amplification procedure is a form of the polymerase chain reaction or a transcription-based amplification.
28. The method of claim 23 further comprising penetrating a seal affixed to the cap with the fluid transfer device, wherein the seal maintains the wick within the cap.
29. The method of claim 19 , wherein the fluid transfer device includes one or more grooves recessed from an outer surface thereof, and wherein at least one of the passageways is formed between at least one of the grooves and the top surface of the cap during the penetrating step.
30. The method of claim 29 , wherein at least one of the grooves overlaps at least one of the ribs on the outer surface of the fluid transfer device.
31. The method of claim 19 , wherein the fluid transfer device is a plastic pipette tip.