Antibodies for enzymes of the ω-oxidation pathway and methods relating thereto
View Patent ↗The present invention provides antigenic peptides useful for the production of antibodies which selectively bind to an enzyme involved in the ω-oxidation of fatty acids and alkanes to α,ω-dicarboxylic acids in yeast. Antibodies which specifically bind to an enzyme involved in the ω-oxidation of fatty acids and alkanes to α,ω-dicarboxylic acids in yeast are also provided. In addition, methods of producing the subject antibodies, a method of detecting the presence and amount of a specific enzyme involved in the ω-oxidation of fatty acids and alkanes to α,ω-dicarboxylic acids, and a method of monitoring the degree of enzyme induction and/or enzyme stability in a mixture during ω-oxidation of fatty acids and alkanes to α,ω-dicarboxylic acids in yeast, are also provided.
1. A method of detecting the presence and amount of a specific enzyme involved in the ω-oxidation of fatty acids and alkanes to α,ω-dicarboxylic acids in a mixture suspected of having said enzyme wherein the specific enzyme comprises the amino acid sequence of SEQ ID NO: 15 and SEQ ID NO: 16, the method comprising:
generating an antibody which specifically binds to the antigenic peptide of SEQ ID NO:15 or the antigenic peptide of about SEQ ID NO:16,
incubating the antibody with the mixture for a time and under conditions sufficient to allow binding of the antibody to the specific ω-hydroxylase enzyme, detecting the enzyme-antibody complex, and determining the amount of the enzyme-antibody complex.
2. The method of claim 1 wherein the enzyme involved in the ω-oxidation of fatty acids and alkanes to α,ω-dicarboxylic acids is cytochrome P450 monooxygenase.