Functionalized nanoparticles and methods of use
View Patent ↗Silica-coated nanoparticles functionalized with biologically active molecules such as antibodies and nucleotides are used to label cells, to detect and isolate nucleic acid molecules having specific nucleotide sequences, and to separate a mixture of different nucleic acid molecules.
1. A functionalized magnetic nanoparticle comprising a metal core and a silica surface, the silica surface being conjugated with at least one functional group comprising an oligonucleotide in the form of a molecular beacon, wherein the sequence of said oligonucleotide comprises a single-stranded loop structure comprising a nucleic acid sequence of interest, and a stem-forming arm structure on either side of the sequence of interest, the two stem-forming arm structures comprising bases complementary to one another that form a stem when hybridized with one another, said oligonucleotide further comprising a fluorophore attached at one end of said sequence, and a quencher attached at the other end of said sequence, wherein fluorescence emission of the fluorphore is inhibited by the quencher when the stem-forming structures are hybridized to one another in the absence of a complement of the nucleic acid sequence of interest.
2. The functionalized nanoparticle of claim 1 , wherein the molecular beacon is conjugated to the silica surface by a biotin-avidin linkage.
3. The functionalized nanoparticle of claim 1 , wherein the sequence of said oligonucleotide differs from the target sequence by one base.
4. A functionalized magnetic nanoparticle comprising a metal core and a silica surface, the silica surface being conjugated with at least one functional group comprising an oligonucleotide in the form of a molecular beacon, wherein the sequence of said oligonucleotide comprises a single-stranded loop structure comprising a nucleic acid sequence of interest and wherein the sequence of said oligonucleotide differs from the target sequence by one or more bases, and a stem-forming arm structure on either side of the sequence of interest, the two stem-forming arm structures comprising bases complementary to one another that form a stem when hybridized with one another, said oligonucleotide further comprising a fluorophore attached at one end of said sequence, and a quencher attached at the other end of said sequence, wherein fluorescence emission of the fluorphore is inhibited by the quencher when the stem-forming structures are hybridized to one another in the absence of a complement of the nucleic acid sequence of interest.