Lipoxygenase proteins and polynucleotides encoding the same
View Patent ↗The nucleotide and corresponding amino acid sequences are reported for a novel class of mammalian lipoxygenase proteins. The novel lipoxygenase encoding polynucleotides were obtained from human gene trap clones and human cDNA libraries.
1. An isolated polynucleotide comprising a first polynucleotide capable of hybridizing to a second polynucleotide in 0.5 M NaHPO 4 , 7% sodium dodecyl sulfate (SDS), 1 mM EDTA at 65° C. with washing in 0.1×SSC/0.1% SDS at 68° C., wherein the first polynucleotide encodes a polypeptide having lipoxygenase activity and wherein the second polynucleotide is complementary to the entire sequence of a polynucleotide with the sequence of SEQ ID NO:1.
2. A vector comprising the isolated polynucleotide of claim 1 .
3. An in vitro host cell comprising the vector of claim 2 .