Removal of adenine during a pathogen reduction process in whole blood or red blood cell by dilution
View Patent ↗The methods of this invention involve preventing the formation of a complex between adenine and riboflavin by reducing the amount of adenine in a solution containing blood or blood components to be pathogen reduced.
1. A method for treating a selected quantity of fluid comprising whole blood to reduce pathogens which may be present therein, the method comprising:
(a) reducing the concentration of adenine in the selected quantity of fluid by adding a sufficient volume of a diluting solution to the selected quantity of fluid;
(b) mixing a pathogen reduction-effective, substantially non-toxic amount of 7,8-dimethyl-10-ribityl isoalloxazine photosensitizer with the fluid;
(c) exposing the fluid containing the photosensitizer to photoradiation of sufficient wavelength and energy to activate the photosensitizer, whereby the pathogens are reduced.
2. A method for treating a selected quantity of fluid to reduce pathogens which may be present wherein the fluid consists essentially of red blood cells, the method comprising:
(a) reducing the concentration of adenine in the selected quantity of fluid by adding a sufficient volume of a diluting solution to the selected quantity of fluid;
(b) mixing a pathogen reduction-effective, substantially non-toxic amount of 7,8-dimethyl-10-ribityl isoalloxazine photosensitizer with the fluid;
(c) exposing the fluid containing the photosensitizer to photoradiation of sufficient wavelength and energy to activate the photosensitizer, whereby the pathogens are reduce.