Bryostatin composition and bryostatin acquisition methodologies
View Patent ↗Methods for obtaining bryostatins from the marine bryozoans of the Bugula neritina species complex are provided wherein the methods involve the extraction and purification of bryostatins from the larvae of Bugula neritina organisms. Further, the present invention relates to a novel bryostatin composition that can be extracted from Bugula neritina.
1. A method of collecting bryostatins, comprising:
subjecting Bugula neritina larvae to a condition sufficient to cause an exudate comprising bryostatin; and
collecting the Bugula neritina larvae exudate.
2. A method of collecting bryostatins, comprising:
collecting larvae of Bugula neritina;
stressing the larvae;
collecting an exudate from the stressed larvae; and
isolating bryostatins from the larvae exudate.
3. The method of claim 2 , wherein collecting larvae of Bugula neritina comprises collecting living larvae of Bugula neritina.
4. The method of claim 2 , wherein collecting larvae of Bugula neritina comprises collecting the larvae in an ocean environment.
5. The method of claim 2 , wherein collecting larvae of Bugula neritina comprises collecting the larvae in a man-made aquacultural environment.
6. The method of claim 2 , wherein collecting larvae of Bugula neritina comprises:
transferring Bugula neritina from an ocean environment to a man-made aquacultural environment; and
collecting larvae from the Bugula neritina in the man-made aquacultural environment.
7. The method of claim 2 , wherein stressing the larvae comprises chemically stressing the larvae.
8. The method of claim 2 , wherein stressing the larvae comprises chemically stressing the larvae with a salt solution selected from the group consisting of magnesium chloride solutions, potassium chloride solutions, and aqueous salt solutions.
9. The method of claim 2 , wherein stressing the larvae comprises physically stressing the larvae.
10. The method of claim 2 , wherein stressing the larvae comprises stressing the larvae to induce the exudation of bryostatins from the larvae.
11. A method of producing bryostatin, comprising:
collecting larvae of Bugula neritina ; and
extracting bryostatin from said larvae.
12. The method of claim 11 , wherein extracting bryostatin from said larvae comprises:
collecting an exudate of the Bugula neritina larvae; and
isolating bryostatins from the exudate.
13. The method of claim 12 , further comprising, stressing the larvae of Bugula neritina to induce the larvae to exude bryostatins.
14. The method of claim 11 , wherein extracting bryostatin from said larvae comprises:
exposing said larvae to a solvent; and
purifying said solvent to obtain bryostatin.
15. A method of harvesting bryostatin from the larvae of Bugula neritina , comprising:
collecting exudate from the larvae of Bugula neritina ; and
isolating bryostatin from the exudate.
16. A purified compound of the formula:
17. A composition comprising a bryostatin compound of the formula:
obtained by a method comprising collecting exudate from the larvae of Bugula neritina.