Radiation-resistant microorganism
View Patent ↗An isolated and purified bacterium is provided which was isolated from a high-level radioactive waste site of mixed waste. The isolate has the ability to degrade a wide variety of organic contaminants while demonstrating high tolerance to ionizing radiation. The organism is uniquely suited to bioremediation of a variety or organic contaminants while in the presence of ionizing radiation.
1. An axenic culture of a microorganism having the ATCC accession number BAA-149, or a mutant thereof, wherein the mutant retains the property of metabolizing a compound selected from the group consisting of aromatic amines, benzene, biphenyl, diphenylamine, organic carbon, phenol, polycyclic aromatics, tetraphenylborate and combinations thereof.
2. A freeze-dried culture of the microorganism having the ATCC accession number BAA-149, or a mutant thereof, wherein the mutant retains the property of metabolizing a compound selected from the group consisting of aromatic amines, benzene, biphenyl, diphenylamine, organic carbon, phenol, polycyclic aromatics, tetraphenylborate and combinations thereof.
3. An isolated pure culture of a microorganism, ATCC BAA-149, or a mutant thereof, wherein said culture and the mutant thereof retain the property of metabolizing a compound selected from the group consisting of aromatic amines, benzene, biphenyl, diphenylamine, organic carbon, phenol, polycyclic aromatics, tetraphenylborate and combinations thereof.
4. A process for biodegradation of a material selected from the group consisting of aromatic amines, benzene, biphenyl, diphenylamine, organic carbon, phenol, polycyclic aromatics, tetraphenylborate and combinations thereof comprising cultivating a microorganism according to claim 1 in an aqueous medium having at least one of said contaminants, said medium further comprising a radioactive environment having radiation levels of at least about 10 Gy per hour.
5. A process for biodegradation of a material selected from the group consisting of aromatic amines, benzene, biphenyl, diphenylamine, organic carbon, phenol, polycyclic aromatics, tetraphenylborate and combinations thereof comprising cultivating a microorganism according to claim 3 in an aqueous medium having at least one of said contaminants, said medium further comprising a radioactive environment having radiation levels of at least about 10 Gy per hour.
6. A process of degrading a contaminant selected from the group consisting of aromatic amines, benzene, biphenyl, diphenylamine, organic carbon, phenol, polycyclic aromatics, tetraphenylborate, and combinations thereof in the presence of ionizing radiation levels of at least about 10 Gy per hour by contacting said contaminant with a microorganism according to claim 1 .
7. A process of degrading a contaminant selected from the group consisting of aromatic amines, benzene, biphenyl, diphenylamine, organic carbon, phenol, polycyclic aromatics, tetraphenylborate, and combinations thereof in the presence of ionizing radiation levels of at least about 10 Gy per hour by contacting said contaminant with a microorganism according to claim 3 .