Plants made insect resistant by transformation with a nucleic acid encoding a modified Cry1Ab protein and methods for making same
View Patent ↗The present invention relates to a DNA sequence encoding a modified Cry1Ab protein that has insecticidal activity. The invention further relates to a method for producing insect resistant plants by introducing into the genome of the plants a foreign DNA comprising such a modified cry1Ab coding sequence. The invention further relates to plants or parts thereof comprising in their genome the modified cry1Ab coding sequence of the present invention.
1. A DNA sequence comprising a coding region encoding a modified Cry1Ab protein, wherein the DNA sequence comprises the nucleotide sequence of SEQ ID NO: 2.
2. An isolated chimeric gene comprising the DNA sequence of claim 1 under control of a plant-expressible promoter.
3. A recombinant vector comprising the chimeric gene of claim 2 .
4. A transgenic plant cell comprising the chimeric gene of claim 2 .
5. A transgenic plant comprising the plant cell of claim 4 .
6. The transgenic plant of claim 5 , which is corn, cotton, or rice.
7. A seed of the plant of claim 6 , wherein the seed comprises the DNA sequence of SEQ ID NO: 2.
8. A method for protecting a plant from lepidopteran insects pests, wherein the method comprises introducing into the plant the chimeric gene of claim 2 in a manner such that the plant produces an insect-controlling amount of the modified Cry1Ab protein.
9. A method for protecting a plant from Lepidopteran insects, comprising growing a plant in a field, wherein said plant produces an insect-controlling amount of a modified insecticidal crystal protein comprising the amino acid sequence of SEQ ID NO: 1 in cells of said plant, wherein the cells comprise a chimeric gene comprising the DNA sequence of SEQ ID NO: 2 under control of a plant-expressible promoter.
10. The method of claim 9 , wherein the cells comprise a chimeric gene comprising the DNA sequence of SEQ ID NO:2 under control of a plant-expressible promoter.