Self aliquoting sample storage plate
View Patent ↗A self-aliquoting dispensing unit for use with a multi-receptacle storage unit is provided including a lower plate having a plurality of access ports therethrough, wherein at least one of the access ports is in fluid communication with a microtube, an upper plate releasably attached to the lower plate, the upper plate including a sample port for supplying a sample to the dispensing unit, and a sealing member for forming a reversible fluid tight connection between the storage unit and the upper plate. An assembly for dispensing a sample into a multi-receptacle storage unit and a method for dispensing a sample into a multi-receptacle storage unit are also provided.
1. A method of dispensing a sample into a storage unit having a plurality of receptacles, said method comprising the steps of:
adding a sample to a dispensing unit, said dispensing unit including:
a lower plate ( 26 ) having a plurality of access ports therethrough, wherein at least one of said access ports is in fluid communication with a tubular microtube ( 32 ) extending from, and having a longitudinal axis disposed transversely to, said lower plate; and
an upper plate ( 28 ) releasably attached to said lower plate, said upper plate including a sample port extending therethrough for supplying a sample to said dispensing unit, wherein said sample port is located spaced from, so as to not overlie, at least a portion of said access ports;
wherein a liquid flow path is defined between, and generally parallel to, said upper and lower plates to permit communication between said sample port and said access ports; and
creating a temperature generated vacuum in said receptacles of said storage unit to dispense an aliquot of said sample into each of said receptacles.
2. The method of claim 1 , further comprising the step of adding a reagent to said storage unit.
3. The method of claim 2 , wherein said step of adding said reagent is performed before adding said sample to said dispensing unit.
4. The method of claim 3 , wherein said reagent is a protease inhibitor.
5. The method of claim 1 , wherein each said access port includes a microtube.
6. The method of claim 1 , wherein said dispensing unit further comprises securement means for securing said lower plate to said upper plate.
7. The method of claim 6 , wherein said securement means comprises:
at least one threaded aperture in said upper plate;
at least one corresponding threaded aperture in said lower plate; and
at least one screw for connecting said apertures.
8. The method of claim 1 , wherein said dispensing unit further comprises a vent hole for allowing fluid communication between said dispensing unit and an ambient environment.
9. The method of claim 8 , wherein said dispensing unit further comprises a vent hole plug for forming a fluid tight seal for said vent hole.
10. The method of claim 1 , wherein said dispensing unit further comprises a separating member on at least one of the upper plate and the lower plate for defining a plurality of sections of said dispensing unit that are fluid tight with respect to one another.
11. The method of claim 1 , wherein said dispensing unit further comprises distribution means interposed between said upper plate and said lower plate for evenly distributing said sample in said dispensing unit.
12. The method of claim 11 , wherein said distribution means comprises a distribution plate having a plurality of uniformly or semi-uniformly spaced hollow channels.
13. The method of claim 12 , wherein said distribution plate further comprises a ramped trough on a top corner of said distribution plate and at least one capillary channel on a bottom side of said distribution plate.
14. The method of claim 1 , wherein said dispensing unit is sterile.
15. The method of claim 1 , wherein each of said receptacles of said storage unit is sealed with a sealing cap.
16. The method of claim 1 , wherein said receptacles of said storage unit are covered with a fluid tight film septum.