Delivery of nucleic acid-like compounds
View Patent ↗A process for preparing a microparticulate complex is provided. The process comprises (a) combining a particle-forming component (“PFC”) and a nucleic acid-like component (“NAC”) in a monophasic composition comprising water and a water-miscible, organic solvent to form a mixture wherein the PFC and the NAC are independently molecularly or micellarly soluble in the aqueous/organic solvent system, and (b) reducing the amount of the organic solvent in the mixture. This effects formation of the microparticulate complex of the NAC and the PFC. Also provided is a microparticulate complex that comprises a particle-forming component complexed to a nucleic acid-like component forming an approximately spherical particle, wherein the particle-forming component encloses an interior of the particle containing the nucleic acid-like component and the so-enclosed interior volume has less than about 50% (preferably less than 20%) of the volume containing free water.
1. A process for preparing a microparticulate complex of a nucleic acid component and a lipid composition, which process comprises:
combining, at a temperature of from 30° to 80° C., the lipid composition, said lipid composition comprising cationic and non-cationic lipids, and the nucleic acid component in an aqueous/organic solvent system consisting essentially of a single liquid phase comprising water and a water-miscible organic solvent in an amount from 40% to about 55% by volume of said liquid phase to form a mixture wherein the lipid composition and the nucleic acid component are independently molecularly or micellarly soluble in said liquid phase, and wherein said organic solvent is an alcohol and is essentially free from a halogenated hydrocarbon, and the ionic strength of the solvent system is less than that of about 50 mM NaCl whereby a monophasic system is formed, and
reducing the amount of the organic solvent in the monophasic system,
whereby the formation of the microparticulate complex of the nucleic acid component and the lipid composition occurs.
2. The process of claim 1 , wherein the water-miscible organic solvent is ethanol.