Compositions for delivering nucleic acids to cells
View Patent ↗Cyclic amidinium containing compounds and their methods of preparation are described. Compositions containing these compounds facilitate delivery of biologically active polymers to cells in vitro and in vivo.
1. A composition for delivering a polynucleotide to a cell comprising: a cationic poly-cyclic amidinium-containing compound capable of condensing nucleic acid and the polynucteotide.
2. The composition of claim 1 wherein the polynucleotide consists of DNA.
3. The composition of claim 1 wherein the polynucleotide consists of RNA.
4. The composition of claim 1 wherein the polynucleotide consists of an siRNA.
5. The composition of claim 1 wherein the poly cyclic amidinium consists of polyimidazolinium.
6. The composition of claim 1 wherein the poly cyclic amidinium consists of a partially acylated polyethylenimine.
7. The composition of claim 6 wherein the partially acylated polyethylenimine consists of an N-propionyl polyethylenimine derivative.
8. The composition of claim 6 wherein the polyethylenimine consists of linear polyethylenimine.
9. The composition of claim 1 wherein the poly cyclic amidinium consists of partially acylated polyallylamine.
10. The composition of claim 1 wherein the poly cyclic amidinium consists of partially acylatcd polyvinylamine.
11. The composition of claim 1 wherein the cell consists of an in vivo cell.
12. The composition of claim 11 wherein the in vivo cell consists of a lung cell.
13. The composition of claim 11 wherein the in vivo cell consists of a liver cell.
14. The composition of claim 13 wherein the liver cell consists of a hepatocyte.
15. A composition for delivering a polynucleotide to a cell comprising: a cationic poly-cyclic amidinium-containing compound capable of condensing nucleic acid, the polynucleotide and a polyanion in a complex wherein the complex has a negative ζ-potential.