Method for producing polymers
The invention relates to a method for producing polymers, in particular synthetic nucleic acid double strands of optional sequence, comprising the steps: (a) providing a support having a surface area which contains a plurality of individual reaction areas, (b) location-resolved synthesizing nucleic acid fragments each having different base sequences in several of the individual reaction areas, and (c) detaching the nucleic acid fragments from individual reaction areas.
1. A method for synthesizing a nucleic acid polymer, the method comprising:
synthesizing in parallel a plurality of different oligomeric nucleic acid building blocks, wherein each of the plurality of different oligomeric nucleic acid building blocks is synthesized in situ bound to a reaction area on a single support, wherein the oligomeric nucleic acid building blocks are partially complementary oligonucleotide building blocks, wherein the plurality of oligomeric building blocks is synthesized on a plurality of reaction areas, wherein the single support comprises multiple channels, wherein each channel comprises one or more reaction spaces for synthesis of oligomeric nucleic acid building blocks and one or more reaction chambers for contacting oligomeric nucleic acid building blocks, wherein each reaction space comprises multiple reaction areas, and wherein each reaction area is on a surface of the channel in the reaction space,
detaching the plurality of oligomeric nucleic acid building blocks from the one or more reaction areas on the single support and
contacting the detached plurality of oligomeric nucleic acid building blocks with one another in multiple reaction chambers to synthesize the nucleic acid polymer, wherein the contacting comprises forming multiple partial nucleic acid polymers that are subsequently combined to form the nucleic acid polymer.
2. The method according to claim 1 , wherein the nucleic acid polymer is a double-stranded nucleic acid polymer of at least 300 bp in length.
3. The method according to claim 1 , wherein the nucleic acid polymer is a nucleic acid selected from the group consisting of a gene or a section thereof, a gene cluster or a section thereof, a chromosome or a section thereof, a viral genome or a section thereof and a bacterial genome or a section thereof.
4. The method according to claim 1 , wherein the oligomeric nucleic acid building blocks are from 5 to 150 monomer units in length.
5. The method according to claim 1 , wherein the the detached partially complementary oligonucleotide building blocks are brought into contact with one another or with an intermediate polymer under hybridization conditions.
6. The method according to claim 1 for producing synthetic nucleic acid double strands of optional sequence, comprising the steps:
(a) providing a support comprising multiple channels comprising a plurality of individual reaction areas in a plurality of reaction spaces,
(b) location-resolved synthesizing nucleic acid fragments each having different base sequences in situ bound to several of the individual reaction areas, and
(c) detaching the nucleic acid fragments from the individual reaction areas.
7. The method according to claim 6 , wherein the base sequences of the nucleic acid fragments synthesized in individual reaction areas are chosen such that they can assemble to form a nucleic acid double strand hybrid.
8. The method according to claim 6 , wherein the nucleic acid fragments according to step (c) are detached in one or more steps under conditions such that a plurality of the detached nucleic acid fragments assemble to form a nucleic acid double strand hybrid.
9. The method according to claim 8 , wherein several nucleic acid fragments which form one strand of the nucleic acid double strand hybrid are linked covalently to one another.
10. The method according to claim 9 , wherein the covalent linking includes treatment with ligase or/and filling in gaps in the strands using DNA polymerase.
11. The method according to claim 6 , wherein the sequence comprises at one or more positions recognition sequences for specific interaction with molecules selected from the group consisting of proteins, nucleic acids, peptides, pharmaceuticals, saccharides, lipids, hormones, and organic compounds.
12. The method according to claim 6 , wherein the sequence of the nucleic acid double strands is selected from the group consisting of a naturally occurring sequence, a non-naturally occurring sequence or a combination of these two.
13. The method according to claim 6 , wherein the sequence is taken from a database, a sequencing experiment or a device for the integrated synthesis and analysis of polymers.
14. The method according to claim 1 , wherein the oligomeric nucleic acid building blocks are synthesized by location- or/and time-resolved illumination by means of a programmable light source matrix.
15. The method according to claim 1 , wherein a location- or/and time-resolved synthesis of the oligomeric nucleic acid building blocks takes place on the reaction areas in the reaction spaces and wherein the single support is a microfluidic reaction support.
16. The method according to claim 1 , wherein the oligomeric building blocks contain nucleotides occurring in nature, modified nucleotides or mixtures thereof.
17. The method according to claim 1 , wherein modified nucleotides are used for labeling and subsequent detection of assembled nucleic acid double strands.
18. The method according to claim 17 , wherein the labeling groups are molecules which are detected in a light-dependent manner.
19. A method for producing a gene product comprising transcribing and translating a nucleic acid polymer obtained by the method according to claim 1 , wherein the nucleic acid polymer is a gene.
20. The method of claim 1 , wherein the nucleic acid polymer is a gene and wherein the method further comprises transcribing and translating the nucleic acid polymer to synthesize a gene product polymer.
21. The method according to claim 1 , wherein the nucleic acid polymer is a double-stranded nucleic acid polymer of at least 1000 by in length.
22. The method according to claim 1 , wherein the nucleic acid oligomeric building blocks are from 5 to 30 monomer units in length.
23. The method according to claim 1 , wherein the one or more reaction spaces are at least partially in fluid communication.
24. The method according to claim 1 , wherein the contacting comprises one or more steps, and wherein at least a portion of the detaching and at least a portion of the contacting are performed within a common channel.
25. The method according to claim 1 , wherein the contacting comprises one or more steps performed in the multiple channels.
26. The method according to claim 1 , wherein multiple channels are at least partially in fluid communication and wherein the contacting comprises one or more steps performed in the multiple channels.
27. The method according to claim 1 , wherein the contacting comprises one or more steps, and wherein at least a portion of the detaching and at least a portion of the contacting are performed within a common channel and wherein the contacting comprises one or more steps performed in the multiple channels.