IP Library › Granted Patent US 7,141,402
Granted Patent B2
US 7,141,402 · App. 10/464,488 · Granted Nov 28, 2006

α-Agarase and process for producing the same

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Quick Facts
Patent No.
US 7,141,402
App. No.
10/464,488
Granted
Nov 28, 2006
Kind
B2
Abstract

A polypeptide having an α-agarase activity, a gene encoding the polypeptide, a method for producing the polypeptide by genetic engineering and a method for producing an agarooligosaccharide using the polypeptide.

Claims (7)

1. An isolated gene encoding an α-agarase, selected from the group consisting of:

(1) a gene encoding a polypeptide containing an amino acid sequence consisting of 749 residues from residue 177 to residue 925 in the amino acid sequence of SEQ ID NO:14;

(2) a gene containing a nucleotide sequence consisting of 2247 bases from nucleotide 529 to nucleotide 2775 in the nucleotide sequence of SEQ ID NO:12; and

(3) a gene that hybridizes to the gene of (2) in a solution containing 6×SSC (1×SSC:0.15M NaCl, 0.015 M sodium citrate, pH 7.0), 0.5% SDS, 5× Denhardt's and 100 mg/ml herring sperm DNA at 65° C.

2. A recombinant DNA molecule which contains the gene defined by claim 1 .

3. An isolated host cell transformed with the recombinant DNA molecule defined by claim 2 .

4. A method for producing α-agarase, comprising culturing the host cell defined by claim 3 and collecting the α-agarase from the culture.