Metabolic gene polynucleotides and polypeptides and uses thereof
View Patent ↗The present invention relates to the field of metabolic research. Metabolic disorders, such as obesity, are a public health problem that is serious and widespread. GMG-3, GMG-4, Cluster 1, GMG-6A, or GMG-6B polypeptides have been identified that are beneficial in the treatment of metabolic disorders. These compounds should be effective for reducing body mass and for treating metabolic-related diseases and disorders. These metabolic-related diseases and disorders include hyperlipidemias, atherosclerosis, diabetes, and hypertension.
1. An isolated polypeptide selected from the group consisting of:
a) a polypeptide comprising SEQ ID NO: 2;
b) a polypeptide comprising amino acids 2-710 of SEQ ID NO: 2 and
c) a polypeptide comprising SEQ ID NO: 2 or amino acids 2-710 of SEQ ID NO: 2 fused to a hcterologous amino acid sequence, wherein the polypeptide of a) or b) or c) increases fatty acid oxidation in muscle cells.
2. A composition comprising a carrier and one or more of the polypeptides of claim 1 .
3. The isolated polypeptide according to claim 1 , wherein said polypeptide comprising SEQ ID NO: 2 or amino acids 2-710 of SEQ ID NO: 2 is fused to a heterologous amino acid sequence.
4. The isolated polypeptide according to claim 3 , wherein said heterologous amino acid sequence is a tag sequence.
5. The isolated polypeptide according to claim 3 , wherein said heterologous amino acid sequence is an Fc fragment.
6. The isolated polypeptide according to claim 1 , wherein said polypeptide comprises amino acids 2-710 of SEQ ID NO: 2.
7. The isolated polypeptide according to claim 1 , wherein said polypeptide comprises SEQ ID NO: 2.
8. The isolated polypeptide according to claim 1 , wherein said heterologous amino acid sequence is a tag sequence and said polypeptide comprises SEQ ID NO: 2.
9. The isolated polypeptide according to claim 1 , wherein said heterologous amino acid sequence is a tag sequence and said polypeptide comprises amino acids 2-710 of SEQ ID NO: 2.
10. The isolated polypeptide according to claim 1 , wherein said heterologous amino acid sequence is an Fc fragment and said polypeptide comprises SEQ ID NO: 2.
11. The isolated polypeptide according to claim 1 , wherein said heterologous amino acid sequence is an Fc fragment and said polypeptide comprises amino acids 2-710 of SEQ ID NO: 2.
12. The composition according to claim 2 , wherein said one or more polypeptides comprises a heterologous amino acid sequence comprising a Fc fragment fused to a polypeptide comprising amino acids 2-710 of SEQ ID NO: 2.
13. The composition according to claim 2 , wherein said one or more polypeptides comprises a heterologous amino acid sequence comprising a tag sequence fused to a polypeptide comprising amino acids 2-710 of SEQ ID NO: 2.
14. The composition according to claim 2 , wherein said one or more polypeptides comprises a heterologous amino acid sequence comprising a Fc fragment fused to a polypeptide comprising SEQ ID NO: 2.
15. The composition according to claim 2 , wherein said one or more polypeptides comprises a heterologous amino acid sequence comprising a Fc fragment fused to a polypeptide comprising amino acids 2-710 of SEQ ID NO: 2.