Methods and means for determining fitness in plants
View Patent ↗The present invention provides methods and means for determining parent inbred plant lines with good combining ability, for determining good combinations of parent inbred plant lines capable of yielding hybrid lines with high heterosis, and further for determining the agronomical performance of different plant lines, which can be performed in vitro by determining the electron flow in the mitochondria under control and stress conditions.
1. A method for determining in vitro the agronomical fitness of a plant line comprising the steps of:
a) culturing a population of explants of said plant line;
b) incubating a first part of said population of cultured explants in a buffer solution;
c) incubating a second part of said population of cultured explants in said buffer solution, further comprising a salicylic acid derivative capable of generating oxidative stress in plant cells incubated in a solution of said salicylic acid derivative, in a concentration of 5 mg/L to 50 mg/L;
d) measuring the electron flow in the mitochondrial electron transport chain in said first and second part of the population; wherein the agronomically fit plants have a lower electron flow in the mitochondrial electron transport chain when assayed under conditions comprising the stress with the salicylic acid derivative then when assayed under control conditions without such imposed stress.
2. The method of claim 1 , wherein said parts of the population of cultured explants are incubated in said buffer solution for about 18 hours.
3. The method of claim 1 or 2 , wherein said buffer solution comprises about 25 mM K-phosphate and about 2 to 3% sucrose.
4. The method of claim 1 , wherein said salicylic acid derivative is acetylsalicylic acid.
5. The method of claim 4 , wherein said acetylsalicylic acid is present in said buffer solution in a concentration of about 25 mM/L.