Method of encoding information in nucleic acids of a genetically engineered organism
View Patent ↗The present invention provides a method of producing a genetically engineered transgenic organism, by (a) incorporating into the organism a functional DNA sequence that confers a trait on an organism and (b) incorporating into the organism a non-functional DNA sequence, wherein the non-functional DNA sequence encodes an information message using a predefined coding scheme, wherein the information message provides information about the functional DNA sequence, and the predefined coding scheme can be used to map a plurality of information messages into a plurality of non-functional DNA sequences and additionally, the functional and the non-functional DNA sequence are incorporated into the same chromosome of the organism.
1. A method of producing a genetically engineered transgenic non-human organism comprising:
(a) incorporating into a non-human organism a functional DNA sequence that confers a trait on said organism; and
(b) incorporating into said organism a non-functional DNA sequence,
wherein said non-functional DNA has no function in the organism other than that of providing information about said functional DNA, further wherein
(i) said non-functional DNA sequence encodes an information message using a predefined coding scheme; and
(ii) said information message provides information about said functional DNA sequence, and
wherein said functional DNA sequence of (a) above and said non-functional DNA sequence of (b) above are incorporated into the same chromosome of said organism, thereby producing a genetically engineered transgenic non-human organism.
2. The method according to claim 1 , wherein said functional DNA sequence is incorporated into said organism prior to said non-functional DNA sequence.
3. The method according to claim 1 , wherein said non-functional DNA sequence is incorporated into said organism prior to said functional DNA sequence.
4. The method according to claim 1 , wherein said non-functional DNA sequence and said functional DNA sequence are incorporated into said organism simultaneously.
5. The method according to claim 1 , wherein said non-functional DNA sequence and said functional DNA sequence are located sufficiently close on said chromosome to prevent separation by recombination.
6. The method according to claim 1 , wherein said non-functional DNA sequence is an intron or encodes an intein.
7. The method according to claim 6 , wherein the intron or intein-encoding sequence is placed within the related functional DNA sequence.
8. The method according to claim 7 , wherein the intron or intein-encoding sequence is placed within a conserved portion of the related functional DNA sequence.
9. The method according to claim 1 , wherein said non-functional DNA sequence, optionally in conjunction with a spacer DNA sequence between said non-functional DNA sequence and said functional DNA sequence, is chosen such that it is not able to form secondary structures and/or such that it is free of recombination or mutation hot spots.
10. The method according to claim 1 , wherein said mapping is from DNA portions to alphanumeric characters.
11. The method according to claim 1 , wherein said predefined coding scheme translates DNA triplets to alphanumeric characters.
12. The method according to claim 1 , wherein said non-functional DNA sequence comprises at least one pre-defined recognition sequence that allows for identification and/or analysis of said non-functional DNA sequence.
13. The method according to claim 12 , wherein said non-functional DNA sequence is flanked at least on one side by the recognition sequence.
14. The method according to claim 12 , wherein said pre defined recognition sequence comprises joint and/or separate recognition of multiple non-functional DNA sequences in the organism.
15. The method according to claim 12 , wherein said pre defined recognition sequences are placed such to allow PCR amplification of said functional DNA sequence or portions thereof using primers complementary to said pre-defined recognition sequences.
16. The method according to claim 12 , wherein said pre defined recognition sequence comprises joint and/or separate recognition of multiple non-functional DNA sequences in the organism.
17. The method according to claim 1 , wherein the functional DNA sequence is flanked on both sides by portions of said non-functional DNA sequence.
18. The method according to claim 1 , wherein said non-functional DNA sequence contains an additional DNA sequence encoding an expressible polypeptide for quick detection.
19. The method according to claim 1 , wherein the information message comprises a trademark, a reference to a database, a date, a place, the name of a producer or an owner, or an indication of the presence and/or position of said functional DNA sequence, or any combination thereof.
20. The method according to claim 1 , wherein said non-functional DNA sequence does not adversely affect the performance of said functional DNA sequence in said genetically engineered organism.
21. The method according to claim 1 , wherein said incorporation into said organism of said non-functional DNA sequence does not adversely affect the genetic stability of said non-functional and/or said functional DNA sequence and/or the incorporation of said non-functional and said functional DNA sequence into the same chromosome of said organism.
22. The method according to claim 1 , wherein the organism is a plant.
23. The method according to claim 1 , wherein the organism is an animal.
24. The method according to claim 1 , wherein said predefined coding scheme can be used to map a plurality of information messages to a plurality of said non-functional DNA sequences.
25. The method according to claim 1 , wherein using said predefined coding scheme, said information message can be non-uniquely mapped to multiple non-functional DNA sequences and said nonfunctional DNA sequence can be uniquely mapped to said information message.