DNA molecules encoding
View Patent ↗The present invention discloses the isolation and characterization of cDNA molecules encoding novel androgen receptor (AR) protein from Macaca mulatta . Also within the scope of the disclosure are recombinant vectors, recombinant host cells, methods of screening for modulators of Macaca mulatta AR (rhAR) activity, purified proteins and fusion proteins which comprise all or a portion of the rhAR protein, transgenic mice comprising a transgene encoding the rhAR protein, as well as production of antibodies against AR, or epitopes thereof.
1. A purified DNA molecule encoding a Macaca mulatta AR protein wherein said protein comprises the amino acid sequence as follows:
MEVQLGLGRV
YPRPPSKTYR
GAFQNLFQSV
REVIQNPGPR
HPEAASAAPP
GASLQQQQQQ
QQETSPRQQQ
QQQQGEDGSP
QAHRRGPTGY
LVLDEEQQPS
QPQSAPECHP
ERGCVPEPGA
AVAAGKGLPQ
QLPAPPDEDD
SAAPSTLSLL
GPTFPGLSSC
SADLKDILSE
ASTMQLLQQQ
QQEAVSEGSS
SGRAREASGA
PTSSKDNYLE
GTSTISDSAK
ELCKAVSVSM
GLGVEALEHL
SPGEQLRGDC
MYAPVLGVPP
AVRPTPCAPL
AECKGSLLDD
SAGKSTEDTA
EYSPFKGGYT
KGLEGESLGC
SGSAAAGSSG
TLELPSTLSL
YKSGALDEAA
AYQSRDYYNF
PLALAGPPPP
PPPPHPHARI
KLENPLDYGS
AWAAAAAQCR
YGDLASLHGA
GAAGPGSGSP
SAAASSSWHT
LFTABEGQLY
GPCGGGGGGG
GGGGGGAGEA
GAVAPYGYTR
PPQGLAGQEG
DFTAPDVWYP
GGMVSRVPYP
SPTCVKSEMG
PWMDSYSGPY
GDMRLETAHD
HVLPIDYYFP
PQKTCLICGD
EASGCHYGAL
TCGSCKVFFK
RAAEGKQKYL
CASRNDCTID
KFRRKIWPSC
RLRKCYEAGM
TLGARKLKKL
GNLKLQEEGE
ASSTTSPTEE
TAQKLTVSHI
EGYECQPIFL
NVLEAIEPGV
VCAGHDNNQP
DSFAALLSSL
NELGERQLVH
VVKWAKALPG
FRNLHVDDQM
AVIQYSWNGL
MVFAMGWRSF
TNVNSRMLYF
APDLVFNEYR
MHKSRMYSQC
VPMRHLSQEF
GWLQITPQEF
LCMKALLLFS
IIPVDGLKNQ
KFFDELRMNY
IKELDRIIAC
KRKNPTSCSR
RFYQLTKLLD
SVQPIARELH
QFTFDLLIKS
HMVSVDFPEM
MARIISVQVP
KILSGKVKPI
YFHTQ,
as set forth in three-letter abbreviation
in SEQ ID NO:2.
2. A DNA expression vector for expressing a Macaca mulatta AR protein in a recombinant host cell wherein said expression vector comprises a DNA molecule of claim 1 .
3. A host cell which expresses a recombinant Macaca mulatta AR protein wherein said host cell contains the DNA expression vector of claim 2 .
4. A process for expressing a Macaca mulatta AR protein in a recombinant host cell, comprising:
(a) transfecting the expression vector of claim 2 into a suitable host cell; and
(b) culturing the host cells of step (a) under conditions which allow expression of said the Macaca mulatta AR protein from said DNA expression vector.
5. A purified DNA molecule encoding a Macaca mulatta AR protein wherein said protein consists of the amino acid sequence as follows:
MEVQLGLGRV
YPRPPSKTYR
GAFQNLFQSV
REVIQNPGPR
HPEAASAAPP
GASLQQQQQQ
QQETSPRQQQ
QQQQGEDGSP
QAHRRGPTGY
LVLDEEQQPS
QPQSAPECHP
ERGCVPEPGA
AVAAGKGLPQ
QLPAPPDEDD
SAAPSTLSLL
GPTFPGLSSC
SADLKDILSE
ASTMQLLQQQ
QQEAVSEGSS
SGRAREASGA
PTSSRDNYLE
GTSTISDSAK
ELCKAVSVSM
GLGVEALEHL
SPGEQLRGDC
MYAPVLGVPP
AVRPTPCAPL
AECKGSLLDD
SAGKSTEDTA
EYSPFKGGYT
KGLEGESLGC
SGSAAAGSSG
TLELPSTLSL
YKSGALDEAA
AYQSRDYYNF
PLALAGPPPP
PPPPHPHARI
KLENPLDYGS
AWAAAAAQCR
YGDLASLHGA
GAAGPGSGSP
SAAASSSWHT
LFTAEEGQLY
GPCGGGGGGG
GGGGGGAGEA
GAVAPYGYTR
PPQGLAGQEG
DFTAPDVWYP
GGMVSRVPYP
SPTCVKSEMG
PWMDSYSGPY
GDMRLETAPD
HVLPIDYYFP
PQKTCLICGD
EASGCHYGAL
TCGSCKVFFK
RAAEGKQKYL
CASRNDCTID
KFRRKNCPSC
RLRKCYEAGM
TLGARKLKKL
GNLKLQEEGE
ASSTTSPTEE
TAQKLTVSHI
EGYECQPIFL
NVLEAIEPGV
VCAGHDNNQP
DSFAALLSSL
NELGERQLVH
VVKWAKALPG
FRNLHVDDQM
AVIQYSWMGL
MVFAMGWRSF
TNVNSRMLYF
APDLVFNEYR
MHKSRMYSQC
VRMRHLSQEF
GWLQITPQEF
LCMKALLLFS
IIPVDGLKNQ
KFFDELRMNY
IKELDRIIAC
KRKNPTSCSR
RFYQLTKLLD
SVQPIARELH
QFTFDLLIKS
HMVSVDFPEM
MAEIISVQVP
KILSGKVKPI
YFHTQ,
as set forth in three-letter abbreviation in SEQ ID NO:2.
6. A DNA expression vector for expressing a Macaca mulatta AR protein in a recombinant host cell wherein said expression vector comprises a DNA molecule of claim 5 .
7. A host cell which expresses a recombinant Macaca mulatta AR protein wherein said host cell contains the expression vector of claim 6 .
8. A process for expressing a Macaca mulatta AR protein in a recombinant host cell, comprising:
(a) transfecting the expression vector of claim 6 into a suitable host cell; and
(b) culturing the host cells of step (a) under conditions which allow expression of said the Macaca mulatta AR protein from said expression vector.
9. A purified DNA molecule encoding a Macaca mulatta AR protein wherein said DNA molecule comprises the nucleotide sequence, as follows:
CCCAAAAAAT
AAAAACAAAC
AAAAACAAAA
CAAAACAAAA
AAAACGAATA
AAGAAAAAGG
TAATAACTCA
GTTCTTATTT
GCACCTACTT
CCAGTGGACA
CTGAATTTGG
AAGGTGGAGG
ATTCTTGTTT
TTTCTTTTAA
GATCGGGCAT
CTTTTGAATC
TACCCCTCAA
GTGTTAAGAG
ACAGACTGTG
AGCCTAGCAG
GGCAGATCTT
GTCCACCGTG
TCTCTTCTTT
TGCAGGAGAC
TTTGAGGCTG
TCAGAGCGCT
TTTTGCGTGG
TTGCTCCCGC
AAGTTTCCTT
CTCTGGAGCT
TCCCGCAGGT
GGGCAGCTAG
CTGCAGCGAC
TACCGCATCA
TCACAGCCTG
TTGAACTCTT
CTGAGCAAGA
GAAGGGGAGG
CGGGGTAAGG
GAAGTAGGTG
GAAGATTCAG
CCAAGCTCAA
GGATGGACGT
GCAGTTAGGG
CTGGGGAGGG
TCTACCCTCG
GCCGCCGTCC
AAGACCTACC
GAGGAGCTTT
CCAGAATCTG
TTCCAGAGCG
TGCGCGAAGT
GATCCAGAAC
CCGGGCCCCA
GGCACCCAGA
GGCCGCGAGC
GCAGCACCTC
CCGGCGCCAG
TTTGCAGCAG
CAGCAGCAGC
AGCAGCAAGA
AACTAGCCCC
CGGCAACAGC
AGCAGCAGCA
GCAGGGTGAG
GATGGTTCTC
CCCAAGCCCA
TCGTAGAGGC
CCCACAGGCT
ACCTGGTCCT
GGATGAGGAA
CAGCAGCCTT
CACAGCCTCA
GTCAGCCCCG
GAGTGCCACC
CCGAGAGAGG
TTGCGTCCCA
GAGCCTGGAG
CCGCCGTGGC
CGCCGGCAAG
GGGCTGCCGC
AGCAGCTGCC
AGCACCTCCG
GACGAGGATG
ACTCAGCTGC
CCCATCCACG
TTGTCTCTGC
TGGGCCCCAC
TTTCCCCGGC
TTAAGCAGCT
GCTCCGCCGA
CCTTAAAGAC
ATCCTGAGCG
AGGCCAGCAC
CATGCAACTC
CTTCAGCAAC
AGCAGCAGGA
AGCAGTATCC
GAAGGCAGCA
GCAGCGGGAG
AGCGAGGGAG
GCCTCGGGGG
CTCCCACTTC
CTCCAAGGAC
AATTACTTAG
A GGGCACTTC
GACCATTTCT
GACAGCGCCA
AGCAGCTGTG
TAAGGCAGTG
TCGGTGTCCA
TGGGCTTGGG
TGTGGAGGCG
TTGGAGCATC
TGAGTCCAGG
GGAACAGCTT
CGGGGGGATT
GCATGTACGC
CCCAGTTTTG
GGAGTTCCAC
CCGCTGTGCG
TCCCACTCCG
TGTGCCCCAT
TGGCCGAATG
CAAAGGTTCT
CTGCTAGACG
ACAGCGCAGG
CAAGAGCACT
GAAGATACTG
CTGAGTATTC
CCCTTTCAAG
GGAGGTTACA
CCAAAGGGCT
AGAAGGCGAG
AGCCTAGGCT
GCTCTGGCAG
CGCTGCAGCA
GGGAGCTCCG
GGACACTTGA
ACTGCCGTCC
ACCCTGTCTC
TCTACAAGTC
CGGAGCACTG
GACGAGGCAG
CTGCGTACCA
GAGTCGCGAC
TACTACAACT
TTCCACTGGC
TCTGGCCGGG
CCGCCGCCCC
CTCCACCGCC
TCCCCATCCC
CACGCTCGCA
TCAAGCTGGA
GAACCCGCTG
GACTATGGCA
GCGCCTGGGC
GGCTGCGGCG
GCGCAGTGCC
GCTATGGGGA
CCTGGCGAGC
CTGCATGGCG
CGGGTGCAGC
GGGACCCGGC
TCTGGGTCAC
CCTCAGCGGC
CGCTTCCTCA
TCCTGGCACA
CTCTCTTCAC
AGCCGAAGAA
GGCCAGTTGT
ATGGACCGTG
TGGTGGTGGG
GGCGGCGGCG
GTGGCGGCGG
CGGCGGCGGC
GCAGGCGAGG
CGGGAGCTGT
AGCCCCCTAC
GGCTACACTC
GGCCACCTCA
GGGGCTGGCG
GGCCAGGAAG
GCGACTTCAC
CGCACCTGAT
GTGTGGTACC
CTGGCGGCAT
GGTGAGCAGA
GTGCCCTATC
CCAGTCCCAC
TTGTGTCAAA
AGCGAGATGG
GCCCCTGGAT
GGATAGCTAC
TCCGGACCTT
ACGGGGACAT
GCGTTTGGAG
ACTGCCAGGG
ACCATGTTTT
GCCAATTGAC
TATTACTTTC
CACCCCAGAA
GACCTGCCTG
ATCTGTGGAG
ATGAAGCTTC
TGGGTGTCAC
TATGGAGCTC
TCACATGTGG
AAGCTGCAAG
GTCTTCTTCA
AAAGAGCCGC
TGAAGGGAAA
CAGAAGTACC
TGTGTGCCAG
CAGAAATGAT
TGCACTATTG
ATAAATTCCG
AAGGAAAAAT
TGTCCATCTT
GCCGTCTTCG
GAAATGTTAT
GAAGCAGGGA
TGACTCTGGG
AGCCCGGAAG
CTGAAGAAAC
TTGGTAATCT
GAAACTACAG
GAGGAAGGAG
AGGCTTCCAG
CACCACCAGC
CCCACTGAGG
AGACAGGCCA
GAAGCTGACA
GTGTCACACA
TTGAAGGCTA
TGAATGTCAG
CCCATCTTTC
TGAATGTCCT
GGAGGCCATT
GAGCCAGGTG
TGGTGTGTGC
TGGACATGAC
AACAACCAGC
CCGACTCCTT
CGCAGCCTTG
CTCTCTAGCC
TCAATGAACT
GGGAGAGAGA
CAGCTTGTAC
ATGTGGTCAA
GTGGGCCAAG
GCCTTGCCTG
GCTTCCGCAA
CTTACACGTG
GACGACCAGA
TGGCTGTCAT
TCAGTACTCC
TGGATGGGGC
TCATGGTGTT
TGCCATGGGC
TGGCGATCCT
TCACCAATGT
CAACTCCAGG
ATGCTCTACT
TTGCCCCTGA
TCTGGTTTTC
AATGAGTACC
GCATGCACAA
ATCCCGGATG
TACAGCCAGT
GTGTCCGAAT
GAGGCACCTC
TCTCAAGAGT
TTGGATGGCT
CCAAATCACC
CCCCAGGAAT
TCCTGTGCAT
GAAAGCGCTG
CTACTCTTCA
GCATTATTCC
AGTGGATGGG
CTGAAAAATC
AAAAATTCTT
TGATGAACTT
CGAATGAACT
ACATCAAGGA
ACTCGATCGT
ATCATTGCAT
GCAAAAGAAA
AAATCCCACA
TCCTGCTCAA
GGCGTTTCTA
CCAGCTCACC
AAGCTCCTGG
ACTCCGTGCA
GCCTATTGCG
AGAGAGCTGC
ATCAGTTCAC
TTTTGACCTG
CTAATCAAGT
CACACATGGT
GAGCGTGGAC
TTTCCGGAAA
TGATGGCAGA
GATCATCTCT
GTGCAAGTGC
CCAAGATCCT
TTCTGGGAAA
GTCAAGCCCA
TCTATTTCCA
CACCCAGTGA
AGCATTGGAA
ATCCCTATTT
CCTCACCCCA
GCTCATGCCC
CCTTTCAGAT
GTCTTCTGCC
TGTTA,
set forth as SEQ ID NO:1.
10. A DNA molecule of claim 9 which consists of nucleotide 154 to about nucleotide 1257 of SEQ ID NO: 1.
11. An expression vector for expressing a Macaca mulatta AR protein wherein said expression vector comprises a DNA molecule of claim 9 .
12. An expression vector for expressing a Macaca mulatta AR protein wherein said expression vector comprises a DNA molecule of claim 10 .
13. A host cell which expresses a recombinant Macaca mulatta AR protein wherein said host cell contains the expression vector of claim 11 .
14. A host cell which expresses a recombinant Macaca mulatta AR protein wherein said host cell contains the expression vector of claim 12 .
15. A process for expressing a Macaca mulatta AR protein in a recombinant host cell, comprising:
(a) transfecting the expression vector of claim 11 into a suitable host cell; and,
(b) culturing the host cells of step (a) under conditions which allow expression of said the Macaca mulatta AR protein from said expression vector.
16. The process of claim 15 wherein the host cell is a yeast host cell.
17. A purified DNA molecule encoding a Macaca mulatta AR protein wherein said DNA molecule consists of the nucleotide sequence, as follows,
CCCAAAAAAT
AAAAACAAAC
AAAAACAAAA
CAAAACAAAA
AAAACGAATA
AAGAAAAAGG
TAATAACTCA
GTTCTTATTT
GCACCTACTT
CCAGTGGACA
CTGAATTTGG
AAGGTGGAGG
ATTCTTGTTT
TTTCTTTTAA
GATCGGGCAT
CTTTTGAATC
TACCCCTCAA
GTGTTAAGAG
ACAGACTGTG
AGCCTAGCAG
GGCAGATCTT
GTCCACCGTG
TGTCTTCTTT
TGCAGGAGAC
TTTGAGGCTG
TCAGAGCGCT
TTTTGCGTGG
TTGCTCCCGC
AAGTTTCCTT
CTCTGGAGCT
TCCCGCAGGT
GGGCAGCTAG
CTGCAGCGAC
TACCGCATCA
TCACAGCCTG
TTGAACTCTT
CTGAGCAAGA
GAAGGGGAGG
CGGGGTAAGG
GAAGTAGGTG
GAAGATTCAG
CCAAGCTCAA
GGATGGAGGT
GCAGTTAGGG
CTGGGGAGGG
TCTACCCTCG
GCCGCCGTCC
AAGACCTACC
GACGAGCTTT
CCAGAATCTG
TTCCAGAGCG
TGCGCGAAGT
GATCCAGAAC
CCGGGCCCCA
GGCACCCAGA
GGCCGCGAGC
GCAGCACCTC
CCGGCGCCAG
TTTGCAGCAG
CAGCAGCAGC
AGCAGCAAGA
AACTAGCCCC
CGGCAACAGC
AGCAGCAGCA
GCAGGGTGAG
GATGGTTCTC
CCCAAGCCCA
TCGTAGAGGC
CCCACAGGCT
ACCTGGTCCT
GGATGAGGAA
CAGCAGCCTT
CACAGCCTCA
GTCAGCCCCG
GAGTGCCACC
CCGAGAGAGG
TTGCGTCCCA
GAGCCTGGAG
CCGCCGTGGC
CGCCGGCAAG
GGGCTGCCGC
AGCAGCTGCC
AGCACCTCCG
GACGAGGATG
ACTCAGCTGC
CCCATCCACG
TTGTCTCTGC
TGGGCCCCAC
TTTCCCCGGC
TTAAGCAGCT
GCTCCGCCGA
CCTTAAAGAC
ATCCTGAGCG
AGGCCAGCAC
CATGCAACTC
CTTCAGCAAC
AGCAGCAGGA
AGCAGTATCC
GAAGGCAGCA
GCAGCGGGAG
AGCGAGGGAG
GCCTCGGGGG
CTCCCACTTC
CTCCAAGGAC
AATTACTTAG
A GGGCACTTC
GACCATTTCT
GACAGCGCCA
AGGAGCTGTG
TAAGGCAGTG
TCGGTGTCCA
TGGGCTTGGG
TGTGGAGGCG
TTGGAGCATC
TGAGTCCAGG
GGAACAGCTT
CGGGGGGATT
GCATGTACGC
CCCAGTTTTG
GGAGTTCCAC
CCGCTGTGCG
TCCCACTCCG
TGTGCCCCAT
TGGCCGAATG
CAAAGGTTCT
CTGCTAGACG
ACAGCGCAGG
CAAGAGCACT
GAAGATACTG
CTGAGTATTC
CCCTTTCAAG
GGAGGTTACA
CCAAAGGGCT
AGAAGGCGAG
AGCCTAGGCT
GCTCTGGCAG
CGCTGCAGCA
GGGAGCTCCG
GGACACTTGA
ACTGCCGTCC
ACCCTGTCTC
TCTACAAGTC
CGGAGCACTG
GACGAGGCAG
CTGCGTACCA
GAGTCGCGAC
TACTACAACT
TTCCACTGGC
TCTGGCCGGG
CCGCCGCCCC
CTCCACCGCC
TCCCCATCCC
CACGCTCGCC
TCAAGCTGGA
GAACCCGCTG
GACTATGGCA
GCGCCTGGGC
GGCTGCGGCG
GCGCAGTGCC
GCTATGGGGA
CCTGGCGAGC
CTGCATGGCG
CGGGTGCAGC
GGGACCCGGC
TCTGGGTCAC
CCTCAGCGGC
CGCTTCCTCA
TCCTGGCACA
CTCTCTTCAC
AGCCGAAGAA
GGCCAGTTGT
ATGGACCGTG
TGGTGGTGGG
GGCGGCGGCG
GTGGCGGCGG
CGGCGGCGGC
GCAGGCGAGG
CGGGAGCTGT
AGCCCCCTAC
GGCTACACTC
GGCCACCTCA
GGGGCTGGCG
GGCCAGGAAG
GCGACTTCAC
CGCACCTGAT
GTGTGGTACC
CTGGCGGCAT
GGTGAGCAGA
GTGCCCTATC
CCAGTCCCAC
TTGTGTCAAA
AGCGAGATGG
GCCCCTGGAT
GGATAGCTAC
TCCGGACCTT
ACGGGGACAT
GCGTTTGGAG
ACTGCCAGGG
ACCATGTTTT
GCCAATTGAC
TATTACTTTC
CACCCCAGAA
GACCTGCCTG
ATCTGTGGAG
ATGAAGCTTC
TGGGTGTCAC
TATGGAGCTC
TCACATGTGG
AAGCTGCAAG
GTCTTCTTCA
AAAGAGCCGC
TGAAGGGAAA
CAGAAGTACC
TGTGTGCCAG
CAGAAATGAT
TGCACTATTG
ATAAATTCCG
AAGGAAAAAT
TGTCCATCTT
GCCGTCTTCG
GAAATGTTAT
GAAGCAGGGA
TGACTCTGGG
AGCCCGGAAG
CTGAAGAAAC
TTGGTAATCT
GAAACTACAG
GAGGAAGGAG
AGGCTTCCAG
CACCACCAGC
CCCACTGAGG
AGACAGCCCA
GAAGCTGACA
GTGTCACACA
TTGAAGGCTA
TGAATGTCAG
CCCATCTTTC
TGAATGTCCT
GGAGGCCATT
GAGCCAGGTG
TGGTGTGTGC
TGGACATGAC
AACAACCAGC
CCGACTCCTT
CGCAGCCTTG
CTCTCTAGCC
TCAATGAACT
GGGAGAGAGA
CAGCTTGTAC
ATGTGGTCAA
GTGGGCCAAG
GCCTTGCCTG
GCTTCCGCAA
CTTACACGTG
GACGACCAGA
TGGCTGTCAT
TCAGTACTCC
TGGATGGGGC
TCATGGTGTT
TGCCATGGGC
TGGCGATCCT
TCACCAATGT
CAACTCCAGG
ATGCTCTACT
TTGCCCCTGA
TCTGGTTTTC
AATGAGTACC
GCATGCACAA
ATCCCGGATG
TACAGCCAGT
GTGTCCGAAT
GAGGCACCTC
TCTCAAGAGT
TTGGATGGCT
CCAAATCACC
CCCCACGAAT
TCCTGTGCAT
GAAAGCGCTG
CTACTCTTCA
GCATTATTCC
AGTGGATGGG
CTGAAAAATC
AAAAATTCTT
TGATGAACTT
CGAATGAACT
ACATCAAGGA
ACTCGATCGT
ATCATTGCAT
GCAAAAGAAA
AAATCCCACA
TCCTGCTCAA
GGCGTTTCTA
CCAGCTCACC
AAGCTCCTGG
ACTCCGTGCA
GCCTATTGCG
AGAGAGCTGC
ATCAGTTCAC
TTTTGACCTG
CTAATCAAGT
CACACATGGT
GAGCGTGGAC
TTTCCGGAAA
TGATGGCAGA
GATCATCTCT
GTGCAAGTGC
CCAAGATCCT
TTCTGGGAAA
GTCAAGCCCA
TCTATTTCCA
CACCCAGTGA
AGCATTGGAA
ATCCCTATTT
CCTCACCCCA
GCTCATGCCC
CCTTTCAGAT
GTCTTCTGCC
TGTTA,
as set forth in SEQ ID NO: 1.
18. A DNA molecule of claim 17 which consists of nucleotide 423 to about nucleotide 3108 of SEQ ID NO: 1.
19. A DNA expression vector for expressing a Macaca mulatta AR protein wherein said expression vector comprises a DNA molecule of claim 17 .
20. A DNA expression vector for expressing a Macaca mulatta AR protein wherein said expression vector comprises a DNA molecule of claim 18 .
21. A host cell which expresses a recombinant Macaca mulatta AR protein wherein said host cell contains the expression vector of claim 19 .
22. A host cell which expresses a recombinant Macaca mulatta AR protein wherein said host cell contains the expression vector of claim 20 .
23. A process for expressing a Macaca mulatta AR protein in a recombinant host cell, comprising:
(a) transfecting the expression vector of claim 19 into a suitable host cell; and
(b) culturing the host cells of step (a) under conditions which allow expression of said the Macaca mulatta AR protein from said expression vector.
24. The process of claim 23 wherein the host cell is a yeast host cell.