Rhesus monkey, dog and ferret melanin-concentrating hormone type 2 receptor
View Patent ↗The present invention identifies the presence of MCH-2R in the dog, ferret and rhesus monkey. MCH-2R is a G-protein coupled receptor that responds to MCH and is distinct from MCH-1R. MCH-2R polypeptide and nucleic acid sequences related to the dog, ferret, and rhesus monkey MCH-2R are described herein along with uses of such polypeptides and nucleic acids.
1. A purified polypeptide comprising an amino acid SEQ ID NO: 9.
2. The polypeptide of claim 1 , wherein said polypeptide consists of an MCH-2R dog, ferret, or rhesus sequence comprising SEQ ID NO: 9.
3. The polypeptide of claim 1 , wherein said polypeptide comprises a sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, and SEQ ID NO: 3.
4. The polypeptide of claim 3 , wherein said polypeptide consists of SEQ ID NO: 1.
5. The polypeptide of claim 3 , wherein said polypeptide consists of SEQ ID NO: 2.
6. The polypeptide of claim 3 , wherein said polypeptide consists of SEQ ID NO: 3.
7. A purified nucleic acid comprising a nucleotide sequence selected from the group consisting of: SEQ ID NO: 13 and the complement of SEQ ID NO: 13.
8. A method of measuring the ability of a compound to affect MCH-2R activity comprising the steps of:
a) contacting a recombinant cell with said compound, wherein said recombinant cell comprises a recombinant nucleic acid expressing a functional MCH-2R that comprises the amino acid sequence of either SEQ ID NO: 1 SEQ ID NO: 2 or SEQ ID NO: 3; and
b) measuring MCH-2R activity.
9. The method of claim 8 , wherein said method further comprises the use of an MCH-2R agonist.
10. The method of claim 8 , wherein said amino acid sequence is SEQ ID NO: 3.
11. A recombinant nucleic acid comprising a nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 9.
12. The recombinant nucleic acid of claim 11 , wherein said nucleotide encodes the sequence of either SEQ ID NO: 1, SEQ ID NO: 2, or SEQ ID NO: 3.
13. The recombinant nucleic acid of claim 12 , wherein said recombinant nucleic acid is an expression vector.
14. The recombinant nucleic acid of claim 11 , wherein said nucleotide sequence encodes SEQ ID NO: 3.
15. The recombinant nucleic acid of claim 11 , wherein said nucleotide sequence is selected from the group consisting of: SEQ ID NO: 5, SEQ ID NO: 6, and SEQ ID NO: 7.
16. The recombinant nucleic acid of claim 15 , wherein said nucleotide sequence is NO: 7.
17. A recombinant cell comprising the expression vector of claim 12 , wherein said nucleotide sequence is functionally coupled to a promoter recognized by said cell.
18. A method of preparing a MCH-2R polypeptide comprising the step of growing the recombinant cell of claim 17 under conditions wherein said polypeptide is expressed from said expression vector.