Core-shell particle including signal-generating substance enclosed therein and process for producing the same
View Patent ↗A core-shell particle including a signal-generating substance therein, wherein the core-shell particle consists of (1) a core portion substantially made of a water-insoluble polymer compound; and (2) a shell portion substantially made of a water-soluble polymer compound having a reactive functional group, and covering a surface of the core portion in the manner of bristles of a brush; and the core portion and the shell portion are, as a whole, a block copolymer of a water-insoluble polymer and a water-soluble polymer, characterized in that the signal-generating substance is included in the core portion, is disclosed, and a process for producing the same is also disclosed. The core-shell particle including a signal-generating substance therein is useful as a labeling substance in which neither radioisotopes having handling restrictions nor unstable enzymes are required, a high sensitivity superior to fluorescent substances is achieved, and nonspecific absorption does not occur.
1. A core-shell particle including a signal-generating substance therein, wherein the core-shell particle consists of
(1) a core portion substantially made of a water-insoluble polymer compound; and
(2) a shell portion substantially made of a water-soluble polymer compound having a reactive functional group, and covering a surface of the core portion in the manner of bristles of a brush; and
the core portion and the shell portion are, as a whole, a block copolymer of a water-insoluble polymer and a water-soluble polymer,
characterized in that the signal-generating substance is included in the core portion.
2. The core-shell particle including a signal-generating substance therein according to claim 1 , wherein the water-soluble polymer compound is polyethyleneglycol.
3. The core-shell particle including a signal-generating substance therein according to claim 2 , wherein the water-insoluble polymer compound is polystyrene.
4. The core-shell particle including a signal-generating substance therein according to claim 2 , wherein the reactive functional group is an aldehyde, amino, carboxy, maleimide, or succinimide group.
5. The core-shell particle including a signal-generating substance therein according to claim 2 , wherein the signal-generating substance is a dye.
6. The core-shell particle including a signal-generating substance therein according to claim 2 , wherein the signal-generating substance is a fluorescent substance.
7. The core-shell particle including a signal-generating substance therein according to claim 2 , wherein the signal-generating substance is a lanthanoid chelate.
8. The core-shell particle including a signal-generating substance therein according to claim 1 , wherein the water-insoluble polymer compound is polystyrene.
9. The core-shell particle including a signal-generating substance therein according to claim 8 , wherein the reactive functional group is an aldehyde, amino, carboxy, maleimide, or succinimide group.
10. The core-shell particle including a signal-generating substance therein according to claim 8 , wherein the signal-generating substance is a dye.
11. The core-shell particle including a signal-generating substance therein according to claim 8 , wherein the signal-generating substance is a fluorescent substance.
12. The core-shell particle including a signal-generating substance therein according to claim 8 , wherein the signal-generating substance is a lanthanoid chelate.
13. The core-shell particle including a signal-generating substance therein according to claim 1 , wherein the reactive functional group is an aldehyde, amino, carboxy, maleimide, or succinimide group.
14. The core-shell particle including a signal-generating substance therein according to claim 13 , wherein the signal-generating substance is a dye.
15. The core-shell particle including a signal-generating substance therein according to claim 13 , wherein the signal-generating substance is a fluorescent substance.
16. The core-shell particle including a signal-generating substance therein according to claim 13 , wherein the signal-generating substance is a lanthanoid chelate.
17. The core-shell particle including a signal-generating substance therein according to claim 1 , wherein the signal-generating substance is a dye.
18. The core-shell particle including a signal-generating substance therein according to claim 1 , wherein the signal-generating substance is a fluorescent substance.
19. The core-shell particle including a signal-generating substance therein according to claim 1 , wherein the signal-generating substance is a lanthanoid chelate.
20. A process for producing the core-shell particle including a signal-generating substance therein according to claim 1 , characterized by immersing
(a) a core-shell particle consisting of (1) a core portion substantially made of a water-insoluble polymer compound; and (2) a shell portion substantially made of a water-soluble polymer compound having a reactive functional group, and covering a surface of the core portion in the manner of bristles of a brush; the core portion and the shell portion being a block copolymer of a water-insoluble polymer and a water-soluble polymer as a whole, and
(b) a signal-generating substance in a solution containing an organic solvent capable of swelling the water-insoluble polymer compound, to include the signal-generating substance in the core portion.