Method of promoting nucleic acid transfer
View Patent ↗Means for transferring efficiently a desired nucleic acid into a cell is provided. The present invention comprises using a complex comprising a collagen or a collagen derivative, and a desired nucleic acid.
1. A pharmaceutical preparation for administration to a living body comprising a solution of an electrostatic complex consisting essentially of a double-stranded RNA and a water-soluble atelocollagen, wherein said complex facilitates the transfer of said double-stranded RNA to a target tissue or a target organ and said complex exhibits a major axis of 100 μm or less in length.
2. The preparation according to claim 1 , wherein the double-stranded RNA is 5-30 nucleotides in length.
3. The preparation according to claim 1 , wherein the double-stranded RNA is an RNA derivative.
4. The preparation according to claim 1 , wherein the double-stranded RNA is an RNA comprising a phosphorothioate bond.
5. The preparation according to claim 1 , wherein the double-stranded RNA is an RNA comprising an internucleotide comprising a phosphate, sugar or base moiety chemically modified to avoid enzymatic degradations.
6. A pharmaceutical preparation for administration to a living body comprising a solution of an electrostatic complex consisting essentially of an a double-stranded RNA and a water-soluble atelocollagen, wherein said complex facilitates the transfer of said double-stranded RNA to a target tissue or a target organ and said complex exhibits a major axis of 10 μm or less in length.
7. The preparation according to claim 6 , wherein the double-stranded RNA is 5-30 nucleotides in length.
8. The preparation according to claim 6 , wherein the double-stranded RNA is an RNA derivative.
9. The preparation according to claim 6 , wherein the double-stranded RNA is an RNA comprising a phosphorothioate bond.
10. The preparation according to claim 6 , wherein the double-stranded RNA is an RNA comprising an internucleotide comprising a phosphate, sugar or base moiety chemically modified to avoid enzymatic degradations.