IP Library Granted Patent US 7,300,793
Granted Patent B2
US 7,300,793 · App. 10/483,218 · Granted Nov 27, 2007

Method for preventing the adhesion of particles

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Quick Facts
Patent No.
US 7,300,793
App. No.
10/483,218
Granted
Nov 27, 2007
Kind
B2
Abstract

A method for the prevention of the adhesion of particles, in particular cells and cellular components in solution to surfaces, characterized in that to the solution is added at least one polyalcohol.

Claims (14)

1. A method for inhibiting adhesion of particles to surfaces, said method comprising:

providing the particles comprising at least one of eukaryotic cells, prokaryotic cells, and cellular components thereof;

providing a substrate comprising a microanalysis system or synthetic microparticles, wherein the substrate has surfaces to which the particles are capable of adhering;

providing the particles in a solution comprising polyvinyl alcohol; and

contacting the substrate with the solution, wherein the polyvinyl alcohol is present as a solute in the solution in an amount effective to inhibit adhesion of the particles to the surfaces, wherein the concentration of the polyvinyl alcohol in solution is 0.01% (w/v) to 5% (w/v).

2. A method for inhibiting adhesion of particles to surfaces, said method comprising:

providing the particles comprising at least one of viable eukaryotic cells and viable prokaryotic cells;

providing a substrate comprising a microanalysis system or synthetic microparticles, wherein the substrate has surfaces to which the particles are capable of adhering;

providing the particles in a solution comprising polyvinyl alcohol; and

contacting the substrate with the solution, wherein the polyvinyl alcohol is present as a solute in the solution in an amount effective to inhibit adhesion of the particles to the surfaces;

wherein the concentration of the polyvinyl alcohol in solution is 0.01% (w/v) to 5% (w/v); and

wherein the cells retain their viability.

3. The method according to claim 1 , wherein the surfaces comprise channel surfaces of the microanalysis system.

4. The method according to claim 1 , wherein the surfaces comprise surfaces of the synthetic microparticles.

Assignments (4)
CHANGE OF NAME Recorded Aug 19, 2008
From: EVOTEC TECHNOLOGIES GMBH
To: PERKINELMER CELLULAR TECHNOLOGIES GERMANY GMBH
Reel/Frame 021398/0678 →
CHANGE OF NAME Recorded Jun 29, 2007
From: EVOTEC OAI AG
To: EVOTEC AG
Reel/Frame 019497/0177 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 29, 2007
From: EVOTEC AG
To: EVOTEC TECHNOLOGIES GMBH
Reel/Frame 019497/0225 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 8, 2004
From: GRADL, GABRIELE
To: EVOTEC OAI AG
Reel/Frame 015350/0403 →