Inhibitor of DNA methylation
View Patent ↗Zebularine has hypomethylating activity, and can be used to inhibit, reverse, and/or reduce DNA methylation in vivo and in vitro. Methods are provided for treating methylation-linked conditions through the application of 2-pyrimidinone derivatives, such as Zebularine. Compositions, including pharmaceutical compositions, comprising such derivatives are also provided. Also provided are kits for use in inhibiting DNA methylation, which kits include an amount of a 2-pyrimidinone derivative.
1. A method of reducing, inhibiting or reversing nucleic acid methylation in vivo, comprising:
comparing the uridine-cytidine kinase level and the methylation state of a mammalian tumor tissue sample to the uridine-cytidine kinase level and the methylation state of a mammalian non-tumor tissue sample to determine whether the mammalian tumor tissue sample has an elevated uridine-cytidine kinase level and an elevated methylation state compared to the mammalian non-tumor tissue sample, wherein the mammalian tumor tissue sample and the mammalian non-tumor tissue sample are from the same tissue type;
based on the comparative determination, selecting a mammalian tumor having an elevated uridine-cytidine kinase level and having a hypermethylated DNA sequence that comprises a regulatory region of a tumor suppressor gene; and
reducing, inhibiting or reversing DNA methylation in the mammalian tumor in vivo, wherein the reducing, inhibiting or reversing DNA methylation in the mammalian tumor in vivo consists of administering a hypomethylating effective amount of Zebularine, or Zebularine in combination with tetrahydrouridine, to the mammalian tumor having the elevated level of uridine-cytidine kinase and the hypermethylated DNA sequence.
2. The method of claim 1 , wherein the Zebularine inhibits a DNA methyltransferase.
3. The method of claim 1 , wherein the hypermethylated DNA sequence comprises a CpG dinucleotide.
4. The method of claim 1 , wherein reducing, inhibiting or reversing DNA methylation ameliorates a tumorigenic state of the mammalian tumor having a level of uridine-cytidine kinase elevated above normal levels.
5. The method of claim 1 , wherein the mammalian tumor is selected from the groups consisting of breast, prostate, lung, renal, bladder, squamous cell, ovarian, rectal and metastatic tumors.
6. The method of claim 1 , wherein the tumor suppressor gene is selected from the group consisting of cadherin, estrogen receptor, VHL, H19, 14-3-3 σ, Apaf-1, p53, p16, glutathione-S-transferase, methyl guanine methyltransferase and tissue inhibitor of metalloproteinase-3.