Methods and cell cultures for promoting organogenesis and tissue development
View Patent ↗Methods of promoting liver morphogenesis prior to the functioning of blood vessels by culturing liver cells with endothelial cells is provided. Also provided are cell cultures and method of promoting vasculogenesis of liver tissue by contacting liver cells with endothelial cells.
1. A method of promoting liver differentiation comprising
obtaining hepatic cell precursor in which no vasculature is present;
directly contacting the hepatic cell precursor in vitro with endothelial cells;
culturing the hepatic cell precursor and endothelial cells on a Transwell membrane in culture medium comprising Dulbecco's Modified Eagle Medium, NaHCO 3 , calf serum, and Matrigel thereby promoting liver differentiation and de novo vascular development.
2. A method of promoting de novo vascular development comprising obtaining hepatic cells in which no vasculature is present; directly contacting the hepatic cells in vitro with endothelial cells; and culturing the hepatic cells and endothelial cells; wherein the hepatic cells comprise a hepatic cell line and the endothelial cells comprise human umbilical vein endothelial cells; wherein the hepatic cells and endothelial cells are cultured in a mixture of endothelial cell medium and hepatic cell medium; wherein the endothelial cell medium comprises Endothelial Cell Basal Medium-2, hydrocortisone, hFGF-B, VEGF, R3-IGF-1, Ascorbic acid, heparin, fetal bovine serum, hEGF, and GA-1000; and the hepatic cell medium comprises Dulbecco's Modified Eagle Medium, Ham's F-12 media; fetal calf serum, and insulin.
3. The method of claim 2 , wherein the endothelial cell medium and hepatic cell medium are at a ratio in the range of 1:1 to 20:1.