Methods and materials relating to stem cell growth factor-like polypeptides and polynucleotides
View Patent ↗The invention provides novel polynucleotides and polypeptides encoded by such polynucleotides and mutants or variants thereof that correspond to a novel human secreted stem cell growth factor-like polypeptides. Other aspects of the invention include vectors containing processes for producing novel human secreted stem cell growth factor-like polypeptides, and antibodies specific for such polypeptides.
1. An isolated polypeptide comprising an amino acid sequence which is at least 99% identical to the amino acid sequence selected from the group consisting of SEQ ID NO:18, or 27.
2. An isolated polypeptide comprising an amino acid sequence which is at least 95% identical to the amino acid sequence selected from the group consisting of SEQ ID NO: 18 or 27.
3. An isolated polypeptide comprising the amino acid sequence of SEQ ID NO: 18 or 27.
4. A composition comprising the polypeptide of claims 1 , 2 or 3 and a pharmaceutically acceptable carrier.
5. The polypeptide of claim 3 produced by the process comprising:
a) culturing a host cell comprising a polynucleotide sequence selected from the group consisting of SEQ ID NO: 17 or 26, or the mature protein portion thereof, under conditions sufficient to express the polypeptide in said cell; and
b) isolating the polypeptide from the cell culture or cells in step (a).
6. A method of producing the polypeptide of claim 3 , comprising:
a) culturing a host cell comprising a polynucleotide sequence selected from the group consisting of SEQ ID NO: 17 or 26, or the mature protein portion thereof, under conditions sufficient to express the polypeptide in said cell; and
b) isolating the polypeptide from the cell culture or cells in step (a).
7. An isolated polypeptide encoded by a polynucleotide selected from the group consisting of SEQ ID NO: 17 or 26, or the mature protein coding portion thereof.