Hybrid and tandem expression of neisserial proteins
View Patent ↗Two or more Neisserial proteins are joined such that they are translated as a single polypeptide chain. Hybrid proteins are represented by the formula NH 2 -A-[-X-L-] n -B—COOH where X is an amino acid sequence, L is an optional linker amino acid sequence, A is an optional N-terminal amino acid sequence, B is an optional C-terminal amino acid sequence, and n is an integer greater than 1. Proteins where each of the n -X- moieties shares sequence identity to each other -X- moiety, the protein is a ‘tandem protein’.
1. The hybrid protein comprising formula:
NH 2 -A-[-X-L-] n -B—COOH
wherein L is an optional linker amino acid sequence, A is an optional N-terminal amino acid sequence, B is an optional C-terminal amino acid sequence, and n is an integer greater than 1 and an X 1 portion of the NH 2 -A-[-X-L-] n -B—COOH formula comprises an amino acid sequence having at least 90% identity to SEQ ID NO: 2206 and an X 2 portion of the NH 2 -A-[-X-L-] n -B—COOH formula comprises an amino acid sequence having at least 90% identity to SEQ ID NO: 2207.
2. The hybrid protein of claim 1 wherein n=2.
3. The hybrid protein of claim 1 , wherein L has 20 or fewer amino acids.
4. The hybrid protein of claim 1 , wherein L is a poly-glycine linker.
5. The hybrid protein of claim 4 , wherein the poly-glycine linker is GSGGGG (SEQ ID NO: 27).
6. The hybrid protein of claim 1 , wherein A has 40 or fewer amino acids.
7. The hybrid protein of claim 1 , wherein B has 40 or fewer amino acids.
8. A composition comprising the hybrid protein of claim 1 and a pharmaceutically acceptable carrier.
9. A method of inducing an immune response in a subject, comprising administering to the subject an effective amount of the composition of claim 8 .