Anti-metasin antibodies and their methods of use
View Patent ↗The present invention aims at providing an antibody, by which metastin or its derivative can be quantified specifically with a high sensitivity, a method of detecting/quantifying metastin or its derivative using the antibody, and a diagnostic agent (e.g., a diagnostic for pregnancy) the same. Specifically, an antibody capable of specifically reacting with a partial peptide at the N-terminus or C-terminus of a polypeptide having the amino acid sequence represented by SEQ ID NO: 1 or its derivative, and a method of detecting/quantifying metastin or its derivative using the antibody as well as a diagnostic agent using the same.
1. An isolated monoclonal antibody named KIS-1Ca that is produced by the hybridoma cell line deposited with the International Patent Organisms Depository (IPOD) as accession number FERM BP-7430.
2. An isolated monoclonal antibody wherein the isolated monoclonal antibody:
(i) is capable of specifically reacting with the C-terminal region of SEQ ID NO: 1, wherein the C-terminal region consists of SEQ ID NO: 6,
(ii) has a neutralizing activity to a peptide having the amino acid sequence of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, or SEQ ID NO: 9, and
(iii) is named Kis-1Ca and is produced by the hybridoma cell line deposited with the International Patent Organisms Depository (IPOD) as accession number FERM BP-7430.
3. The antibody according to claim 1 or claim 2 , which is labeled.
4. The hybridoma cell line deposited with the International Patent Organisms Depository (IPOD) as accession number FERM BP-7430.
5. The hybridoma cell line producing the monoclonal antibody according to claim 1 or claim 2 , which is deposited with the International Patent Organisms Depository (IPOD) as accession number FERM BP-7430.