Polypeptide
A screening tool and a screening method for a therapeutic agent for type II diabetes, particularly an agent for alleviating insulin resistance are disclosed. A novel polypeptide, a polynucleotide, a vector, a promoter, and a transformant which are useful in the screening tool and the screening method, are also disclosed.
1. An isolated polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 2 wherein said polypeptide cleaves the insulin-like growth factor binding proteins 3 and 5.
2. The polynucleotide of claim 1 , wherein said polynucleotide comprises the nucleic acid sequence set forth in SEQ ID NO:1.
3. The polynucleotide of claim 1 , wherein said polynucleotide consists of the nucleic acid sequence of SEQ ID NO:1.
4. A vector comprising the polynucleotide according to claim 1 and further comprising a promoter suitable for the expression of the polynucleotide.
5. A vector comprising the polynucleotide according to claim 2 and further comprising a promoter suitable for the expression of the polynucleotide.
6. A transformed host cell comprising the polynucleotide according to claim 1 .
7. A transformed host cell comprising the polynucleotide according to claim 2 .
8. A method for producing a polypeptide comprising the amino acid sequence of SEQ ID NO: 2, said method comprising:
i) culturing the transformed host cell of claim 6 under conditions promoting expression of the polypeptide encoded by said polynucleotide, and
ii) separating the encoded polypeptide from the transformed host cells, whereby the polypeptide is produced.
9. A method for producing a polypeptide comprising the amino acid sequence of SEQ ID NO:2, comprising:
i) culturing a transformed host cell comprising the polynucleotide according to claim 2 under conditions promoting expression of the polypeptide encoded by said polynucleotide, and
ii) separating the encoded polypeptide from the transformed host cells, whereby the polypeptide is produced.