IP Library Granted Patent US 7,253,199
Granted Patent B2
US 7,253,199 · App. 10/491,902 · Granted Aug 7, 2007

Azole derivatives and pharmaceutical compositions containing them

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Quick Facts
Patent No.
US 7,253,199
App. No.
10/491,902
Granted
Aug 7, 2007
Kind
B2
Abstract

The invention provides substituted pyrazolidinone compounds, and methods of treatment and pharmaceutical compositions that utilize or comprise one or more such compounds. Compounds of the invention are useful for a variety of therapies, including treating or preventing various cancers, inflammation, septic shock, preterm labor, infertility, pain, and ischemia, and other diseases and disorders associated with MEK-1 and/or ERK-2 activation.

Claims (144)

1. A compound of the following Formula (I):

wherein each of A 1 , A 2 , and A 4 are carbon and A 3 is nitrogen;

each R is independently halo, nitro, optionally substituted alkyl; optionally substituted alkenyl; optionally substituted alkynyl; optionally substituted heteroalkyl; optionally substituted heteroalkenyl; optionally substituted heteroalkynyl; optionally substituted alkanol; optionally substituted carbocyclic aryl, optionally substituted heteroalicyclic, optionally substituted heteroaromatic, optionally substituted aralkyl; optionally substituted heteroarylalkyl; and optionally substituted heteroalicyclicalkyl;

or two R groups on adjacent ring atoms are taken together with those ring to form a fused alicyclic, heteroalicyclic, carbocyclic aryl or heteraromatic group having from 4 to about 8 ring members;

k is an integer;

Y is selected from the group consisting of CH 2 CH, CH 2 CH(CH 2 ) q , CH 2 CR 7 CH 2 , and a branched alkyl having the formula CH 2 CH(CH 2 ) p (CH 2 ) s , wherein R 7 is H or C 1 -C 6 alkyl, s and p are the same or different and are zero or a positive integer, and q is a positive integer;

W and W′ are each independently a hetero atom; optionally substituted heteroalkyl; optionally substituted heteroalkenyl; and optionally substituted heteroalkynyl;

Z and Z′ are each independently a chemical bond or alkanoyl;

R 1 and R 2 are each independently optionally substituted carbocyclic aryl or optionally substituted heteroaromatic, wherein when R 2 is a carbocyclic aryl and said carbocyclic aryl is a phenyl, said phenyl is substituted; or pharmaceutically acceptable salt thereof, with the exclusion of racemates of 4-methoxy-benzoic acid 1-imidazol-1-yl methyl-2-phenoxy-ethyl ester, 4-chloro benzoic acid 1-imidazol-1-yl methyl-2-phenoxy-ethyl ester, and benzoic acid 1-imidzol-1-yl methyl-2-phenoxy-ethyl ester.

2. A compound of claim 1 wherein the compound is of the following Formula (II):

wherein each of A 1 , A 2 , and A 4 are carbon and A 3 is nitrogen;

each R is independently halo, nitro, optionally substituted alkyl; optionally substituted alkenyl; optionally substituted alkynyl; optionally substituted heteroalkyl; optionally substituted heteroalkenyl; optionally substituted heteroalkynyl; optionally substituted alkanol; optionally substituted carbocyclic aryl, optionally substituted heteroalicyclic, optionally substituted heteroaromatic, optionally substituted aralkyl; optionally substituted heteroarylalkyl; and optionally substituted heteroalicyclicalkyl;

or two R groups on adjacent ring atoms are taken together with those ring to form a fused alicyclic, heteroalicyclic, carbocyclic aryl or heteraromatic group having from 4 to about 8 ring members;

k is an integer;

s and p are the same or different and are zero or a positive integer;

W and W′ are each independently a hetero atom; optionally substituted heteroalkyl; optionally substituted heteroalkenyl; and optionally substituted heteroalkynyl;

Z and Z′ are each independently a chemical bond or alkanoyl;

R 1 and R 2 are each independently optionally substituted carbocyclic aryl or optionally substituted heteroaromatic, wherein when R 2 is a carbocyclic aryl and said carbocyclic aryl is a phenyl, said phenyl is substituted; or pharmaceutically acceptable salt thereof.

3. A compound of claim 1 wherein the compound is of the following Formula (III):

wherein each of A 1 , A 2 , and A 4 are carbon and A 3 is nitrogen;

each R is independently halo, nitro, optionally substituted alkyl; optionally substituted alkenyl; optionally substituted alkynyl; optionally substituted heteroalkyl; optionally substituted heteroalkenyl; optionally substituted heteroalkynyl; optionally substituted alkanol; optionally substituted carbocyclic aryl, optionally substituted heteroalicyclic, optionally substituted heteroaromatic, optionally substituted aralkyl; optionally substituted heteroarylalkyl; and optionally substituted heteroalicyclicalkyl;

or two R groups on adjacent ring atoms are taken together with those ring to form a fused alicyclic, heteroalicylic, carbocyclic aryl or heteraromatic group having from 4 to about 8 ring members;

k is an integer;

q is a positive integer,

R 3 and R 4 are each optionally substituted carbocyclic aryl or optionally substituted heteroaromatic, wherein when R 4 is a carbocyclic aryl and said carbocyclic aryl is a phenyl, said phenyl is substituted;

R 5 is hydrogen, optionally substituted alkyl; optionally substituted alkenyl; optionally substituted alkynyl; optionally substituted heteroalkyl; optionally substituted heteroalkenyl; or optionally substituted heteroalkynyl; optionally substituted carbocyclic aryl or optionally substituted heteroaromatic; or pharmaceutically acceptable salt thereof.

4. A compound of claim 1 wherein the compound is of the following Formula (IV):

wherein each of A 1 , A 2 , and A 4 are carbon and A 3 is nitrogen;

each R is independently halo, nitro, optionally substituted alkyl; optionally substituted alkenyl; optionally substituted alkynyl; optionally substituted heteroalkyl; optionally substituted heteroalkenyl; optionally substituted heteroalkynyl; optionally substituted alkanol; optionally substituted carbocyclic aryl, optionally substituted heteroalicyclic, optionally substituted heteroaromatic, optionally substituted aralkyl; optionally substituted heteroarylalkyl; and optionally substituted heteroalicyclicalkyl;

or two R groups on adjacent ring atoms are taken together with those ring to form a fused alicyclic, heteroalicyclic, carbocyclic aryl or heteraromatic group having from 4 to about 8 ring members;

k is an integer;

q is a positive integer;

R 3 and R 4 are each optionally substituted carbocyclic aryl or optionally substituted heteroaromatic, wherein when R 4 is a carbocyclic aryl and said carbocyclic aryl is a phenyl, said phenyl is substituted;

R 5 is hydrogen, optionally substituted alkyl; optionally substituted alkenyl; optionally substituted alkynyl; optionally substituted heteroalkyl; optionally substituted heteroalkenyl; or optionally substituted heteroalkynyl; optionally substituted carbocyclic aryl or optionally substituted heteroaromatic; or pharmaceutically acceptable salt thereof.

5. A compound of claim 1 wherein the compound is of the following Formula (V), or pharmaceutically acceptable salt thereof:

wherein A 1 is CR 9 with R 9 is H, C 1 -C 6 alkyl, C 1 -C 6 alkyl, aryl or halogen;

B is —C(O)—

R 6 is C 1 -C 6 alkyl, C 3 -C 6 cycloalkyl C 1 -C 6 alkyl, C 3 -C 6 heterocycloalkyl, aryl or heteroaryl;

R 7 is H or C 1 -C 6 alkyl;

R 8 is C 1 -C 6 alkyl, C 1 -C 6 alkoxy or halogen;

n is 0 or 1;

m is 1 or 2.

6. A compound of claim 5 , or pharmaceutically acceptable salt thereof, wherein A 1 is CR 9 with R 9 is H, C 1 -C 6 alkyl, aryl or halogen; R 6 is C 1 -C 6 alkyl, C 3 -C 6 cycloalkyl C 1 -C 6 alkyl, C 3 -C 6 heterocycloalkyl, aryl or heteroaryl; R 7 is H or C 1 -C 6 alkyl; R 8 is C 1 -C 6 alkyl, C 1 -C 6 alkoxy or halogen; n is 0 or 1; m is 1 or 2.

7. A compound of claim 6 , or pharmaceutically acceptable salt thereof, wherein A 1 is CR 9 with R 9 is H, C 1 -C 6 alkyl or halogen; R 6 is aryl or heteroaryl; R 7 is H or C 1 -C 6 alkyl; R 8 is substituted C 1 -C 6 alkyl, C 1 -C 6 alkoxy or halogen; n is 0; m is 1 or 2.

8. A compound of claim 7 , or pharmaceutically acceptable salt thereof, wherein A 1 is CR 9 with R 9 is H, C 1 -C 6 alkyl or halogen; R 6 is aryl or heteroaryl; R 7 is H; R 8 is C 1 -C 6 alkyl, C 1 -C 6 alkoxy or halogen; n is 0; m is 1 or 2.

9. A compound of claim 1 wherein the compound is of the following Formula (VI), or pharmaceutically acceptable salt thereof:

wherein A 1 is CR 9 with R 9 is H, C 1 -C 6 alkyl, C 1 -C 6 alkyl, aryl or halogen;

B is —C(O)—

R 6 is C 1 -C 6 alkyl, C 3 -C 6 cycloalkyl C 1 -C 6 alkyl, C 3 -C 6 heterocycloalkyl, aryl or heteroaryl;

R 7 is H or C 1 -C 6 alkyl;

R 8 is C 1 -C 6 alkyl, C 1 -C 6 alkoxy or halogen;

n is 0 or 1;

m is 1 or 2.

10. A compound of claim 9 , or pharmaceutically acceptable salt thereof, wherein A 1 is CR 9 with R 9 is H, C 1 -C 6 alkyl, aryl or halogen; R 6 is C 1 -C 6 alkyl, C 3 -C 6 cycloalkyl C 1 -C 6 alkyl, C 3 -C 6 heterocycloalkyl, aryl or heteroaryl; R 7 is H or C 1 -C 6 alkyl; R 8 is C 1 -C 6 alkyl, C 1 -C 6 alkoxy or halogen; n is 0 or 1; m is 1 or 2.

11. A compound of claim 10 , or pharmaceutically acceptable salt thereof, wherein A 1 is CR 9 with R 9 is H, C 1 -C 6 alkyl or halogen; R 6 is aryl or heteroaryl; R 7 is H or C 1 -C 6 alkyl; R 8 is substituted C 1 -C 6 alkyl, C 1 -C 6 alkoxy or halogen; n is 0; m is 1 or 2.

12. A compound of claim 3 , or pharmaceutically acceptable salt thereof, wherein q is 1.

13. A compound claim 1 , or pharmaceutically acceptable salt thereof, wherein R 3 and/or R 4 is optionally substituted carbocyclic aryl.

14. A compound of claim 1 , or pharmaceutically acceptable salt thereof, wherein R 3 and/or R 4 is optionally substituted phenyl.

15. A compound of claim 1 , or pharmaceutically acceptable salt thereof, wherein R 3 and/or R 4 is optionally substituted heteroaromatic.

16. A compound of claim 1 , wherein said compound exhibits a single dose inhibition of about less than 1,000 nM in a standard MEK-1 inhibition assay when administered to a subject in need of MEK-1 inhibition.

17. A compound of claim 1 , wherein said compound exhibits a single dose inhibition of about less than 100 nM in a standard MEK-1 inhibition assay when administered to a subject in need of MEK-1 inhibition.

18. A compound of claim 1 wherein the compound is, or pharmaceutically acceptable salt thereof, selected from the group consisting of:

4-methoxy-benzoic acid 1-imidazol-1-yl methyl-2-(4-fluorophenoxy)-ethyl ester;

3-methoxy-benzoic acid 1-imidazol-1-yl methyl-2-phenoxy-ethyl ester;

4-methoxy-benzoic acid 1-imidazol-1-yl methyl-2-(4-methoxy phenoxy)-ethyl ester;

4-methoxy-benzoic acid 1-imidazol-1-yl methyl-2-(4-iodophenoxy)-ethyl ester;

4-iodo-benzoic acid 1-imidazol-1-yl methyl-2-(4-iodo phenoxy)-ethyl ester;

4-bromo-2-chloro-benzoic acid 1-imidazol-1-yl methyl-2-phenoxy)-ethyl ester;

4-bromo-2-chloro-benzoic acid 1-imidazol-1-yl methyl-2-(4-iodophenoxy)-ethyl ester;

4-bromo-benzoic acid 1-imidazol-1-yl methyl-2-phenoxy-ethyl ester;

4-trifluoromethyl-benzoic acid 1-imidazol-1-yl methyl-2-phenoxy-ethyl ester;

N-(1-imidazol-1-yl methyl-2-phenoxy-ethyl)-4-methoxy-beflzamide;

4-iodo-benzoic acid 1-imidazol-1-yl methyl-2-(4-fluorophenoxy)-ethyl ester;

4-fluoro-benzoic acid 1-imidazol-1-yl methyl-2-phenoxy-ethyl ester;

N-[(1-imidazol-1-yl methyl)-2-phenoxy-ethyl]-N-methyl-4-iodo-benzamide;

N-[(1-imidazol-1-yl methyl)-2-(4-fluorophenoxy-ethyl)]-N-methyl-4-iodobenzamide;

4-Iodo-benzoic acid 2-(4-acetylamino-phenoxy)-1-imidazol-1-ylmethyl-ethyl ester (R-isomer);

N-[2-(4-Fluoro-phenoxy)-1-imidazol-1-ylmethyl-ethyl]-4-iodo-benzamide (R-isomer);

N-[2-(4-tert-Butyl-phenoxy)-1-imidazol-1-ylmethyl-ethyl]-4-iodo-benzamide;

4-Iodo-benzoic acid 2-imidazol-1-yl-1-(3-nitro-phenoxymethyl)-ethyl ester (R-isomer);

4-Iodo-benzoic acid 2-imidazol-1-yl-1-(2-nitro-phenoxymethyl)-ethyl ester (R-isomer);

4-Iodo-benzoic acid 1-(4-nitrobenzoyloxymethyl)-2-imidazol-1-yl-1-methyl-ethyl ester (R-isomer);

4-Iodo-benzoic acid 2-imidazol-1-yl-1-(4-nitro-phenoxymethyl)-ethyl ester (R-isomer);

4-Iodo-benzoic acid 1-imidazol-1-ylmethyl-2-methoxy-ethyl ester (R-isomer);

4-Iodo-benzoic acid 1-(2-methyl-imidazol-1-ylmethyl)-2-(tetrahydro-pyran-2-yloxy)-ethyl ester;

4-Iodo-benzoic acid 1-imidazol-1-yl methyl-2-(pyridin-3-yloxy)-ethyl ester (R-isomer);

2,4-Dichloro-benzoic acid 2-(4-fluoro-phenoxy)-1-imidazol-1-ylmethyl-ethyl ester (R-isomer);

4-Iodo-benzoic acid 1-(4-cyano-phenoxymethyl)-2-imidazol-1-yl-ethyl ester;

4-Iodo-benzoic acid 1-(4-cyano-phenoxymethyl)-2-(2-methyl-imidazol-1-yl)-ethyl ester (R-isomer);

2-Chloro-N-[2-(4-fluoro-phenoxy)-1-imidazol-1-ylmethyl-ethyl]-4-iodo-benzamide (R-isomer);

N-[2-(4-Fluoro-phenoxy)-1-(2-methyl-imidazol-1-ylmethyl)-ethyl]-4-iodo-benzamide (R-isomer);

2-Chloro-4-iodo-benzoic acid 1-cyclopropylmethoxymethyl-2-(2-methyl-imidazol-1-yl)-ethyl ester (R-isomer);

N-[1-(2-Chloro-imidazol-1-ylmethyl)-2-(4-fluoro-phenoxy)-ethyl]-4-iodo-benzamide (R-isomer);

N-[2-(4-Fluoro-phenoxy)-1-imidazol-1-ylmethyl-ethyl]-3-iodo-benzamide;

2-Chloro-N-[2-imidazol-1-yl-1-(pyridin-3-yloxymethyl)-ethyl]-4-iodo-benzamide;

N-[2-Imidazol-1-yl-1-(pyridin-3-yloxymethyl)-ethyl]-4-iodo-benzamide;

N-[2-(4-Fluoro-phenoxy)-1-(2-phenyl-imidazol-1-ylmethyl)-ethyl]-4-iodo-benzamide;

N-[1-(2-Chioro-imidazol-1-ylmethyl)-2-(4-cyano-phenoxy)-ethyl]-4iodo-benzamide;

N-[2-Imidazol-1-yl-1-(3-nitro-pyridin-2-yloxymethyl)-ethyl]-4-iodo-benzamide;

N-[2-Imidazol-1-yl-1-(4-nitro-phenoxymethyl)-ethyl]-4-iodo-benzamide;

N-[1-(3-Cyano-pyridin-2-yloxymethyl)-2-imidazol-1-yl-ethyl]-4-iodo-benzamide;

N-[1-(5-Cyano-pyridin-2-yloxymethyl)-2-imidazol-1-yl-ethyl]-4-iodo-benzamide;

N-[1-(4-Cyano-phenoxymethyl)-2-imidazol-1-yl-ethyl]-4-iodo-benzamide;

N-[1-(4-Cyano-phenoxymethyl)-2-(2-methyl-imidazol-1-yl)-ethyl]-4-iodo-benzamide; and

N-[1-(2-Difluoromethyl-imidazol-1-ylmethyl)-2-(4-fluoro-phenoxy)-ethyl]-4-iodo-benzamide.

19. A compound of claim 1 wherein the compound is 4-iodo-benzoic acid 1-imidazol-1-yl methyl-2-phenoxy-ethyl ester, or a pharmaceutically acceptable salt thereof.

20. A compound of claim 1 wherein the compound is N-[(1-imidazol-1-yl methyl)-2-phenoxy-ethyl]-4-iodo-benzamide, or a pharmaceutically acceptable salt thereof.

21. A compound of claim 1 , wherein the compound is present in an enantiomeric excess.

22. A compound that is an enantiomeric excess of:

benzoic acid 1-imidazol-1-yl methyl-2-phenoxy-ethyl ester; 4-methoxy-benzoic acid 1-imidazol-1-yl methyl-2-phenoxy-ethyl ester; 4-chloro-benzoic acid 1-imidazol-1-yl methyl-2-phenoxy-ethyl ester; or a pharmaceutically acceptable salt thereof.

23. A pharmaceutical composition comprising a pharmaceutically acceptable carrier and one or more compounds of claim 1 .

24. A commercial product packaged for treatment of cancer, inflammation, septic shock, premature labor, infertility, pain or an ischemia related condition, wherein said commercial product comprises the composition of claim 23 packaged together with instructions for said treatment.

25. A pharmaceutical composition comprising a pharmaceutically acceptable carrier and one or more compounds of the following Formula (I):

wherein each of A 1 , A 2 , and A 4 is are carbon and A 3 is nitrogen;

each R is independently halo, nitro, optionally substituted alkyl; optionally substituted alkenyl; optionally substituted alkynyl; optionally substituted heteroalkyl; optionally substituted heteroalkenyl; optionally substituted heteroalkynyl; optionally substituted alkanol; optionally substituted carbocyclic aryl, optionally substituted heteroalicyclic, optionally substituted heteroaromatic, optionally substituted aralkyl; optionally substituted heteroarylalkyl; and optionally substituted heteroalicyclicalkyl;

or two R groups on adjacent ring atoms are taken together with those ring to form a fused alicyclic, heteroalicyclic, carbocyclic aryl or heteraromatic group having from 4 to about 8 ring members;

k is an integer;

Y is selected from the group consisting of CH 2 CH, CH 2 CH(CH 2 ) q , CH 2 CR 7 CH 2 , and a branched alkyl having the formula CH 2 CH(CH 2 ) p (CH 2 ) s , wherein R 7 is H or C 1 -C 6 alkyl, s and p are the same or different and are zero or a positive integer, and q is a positive integer;

W and W′ are each independently a hetero atom; optionally substituted heteroalkyl; optionally substituted heteroalkenyl; and optionally substituted heteroalkynyl;

Z and Z′ are each independently a chemical bond or alkanoyl;

R 1 and R 2 are each independently optionally substituted carbocyclic aryl or optionally substituted heteroaromatic; or pharmaceutically acceptable salt thereof.

26. A pharmaceutical composition comprising a pharmaceutically acceptable carrier and one or more compounds according to claim 5 .

27. A commercial product packaged for treatment of cancer, inflammation, septic shock, premature labor, infertility, pain or an ischemia related condition, wherein said commercial product comprises the composition of claim 25 packaged together with instructions for said treatment.

28. A compound of claim 1 wherein the compound is of the following Formula (VII), or a pharmaceutically acceptable salt thereof:

wherein A 4 is CR 9 with R 9 is H, C 1 -C 6 alkyl, C 1 -C 6 alkyl, aryl or halogen;

B is —C(O)—R 6 is C 1 -C 6 alkyl, C 3 -C 6 cycloalkyl C 1 -C 6 alkyl, C 3 -C 6 heterocycloalkyl, aryl or heteroaryl;

R 7 is H or C 1 -C 6 alkyl;

R 8 is C 1 -C 6 alkyl, C 1 -C 6 alkoxy or halogen; n is 0 or 1;

m is 1 or 2.

29. A compound of claim 28 , or a pharmaceutically acceptable salt thereof, wherein A 4 is CR 9 with R 9 is H, C 1 -C 6 alkyl, aryl or halogen; R 6 is C 1 -C 6 alkyl, C 3 -C 6 cycloalkyl C 1 -C 6 alkyl, C 3 -C 6 heterocycloalkyl, aryl or heteroaryl; R 7 is H or C 1 -C 6 alkyl; R 8 is C 1 -C 6 alkyl, C 1 -C 6 alkoxy or halogen; n is 0 or 1; m is 1 or 2.

30. A compound of claim 29 , or a pharmaceutically acceptable salt thereof, wherein A 4 is CR 9 with R 9 is H, C 1 -C 6 alkyl or halogen; R 6 is aryl or heteroaryl; R 7 is H or C 1 -C 6 alkyl; R 8 is C 1 -C 6 alkyl, C 1 -C 6 alkoxy or halogen; n is 0 or 1; m is 1 or 2.

31. A compound of claim 30 , or a pharmaceutically acceptable salt thereof, wherein A 4 is CR 9 with R 9 is H, C 1 -C 6 alkyl or halogen; R 6 is aryl or heteroaryl; R 7 is H; R 8 is C 1 -C 6 alkyl, C 1 -C 6 alkoxy or halogen; n is 0; m is 1 or 2.

32. A compound of claim 1 wherein the compound is of the following Formula (VIII), or pharmaceutically acceptable salt thereof:

wherein A 4 is CR 9 with R 9 is H, C 1 -C 6 alkyl, C 1 -C 6 alkyl, aryl or halogen;

B is —C(O)—R 6 is C 1 -C 6 alkyl, C 3 -C 6 cycloalkyl C 1 -C 6 alkyl, C 3 -C 6 heterocycloalkyl, aryl or heteroaryl;

R 7 H or C 1 -C 6 alkyl;

R 8 is C 1 -C 6 alkoxy or halogen;

n is 0 or 1;

m is 1 or 2.

33. A compound of claim 32 , or pharmaceutically acceptable salt thereof, wherein A 4 is CR 9 with R 9 is H, C 1 -C 6 alkyl, aryl or halogen; R 6 is C 1 -C 6 alkyl, C 3 -C 6 cycloalkyl C 1 -C 6 alkyl, C 3 -C 6 heterocycloalkyl, aryl or heteroaryl; R 7 is H or C 1 -C 6 alkyl; R 8 is C 1 -C 6 alkyl or halogen; n is 0 or 1; m is 1 or 2.

34. A compound of claim 33 , or pharmaceutically acceptable salt thereof, wherein A 4 is CR 9 with R 9 is H, C 1 -C 6 alkyl or halogen; R 6 is aryl or heteroaryl; R 7 is H or C 1 -C 6 alkyl; R 8 is C 1 -C 6 alkyl, C 1 -C 6 alkoxy or halogen; n is 0 or 1; m is 1 or 2.

35. A compound of claim 2 , or pharmaceutically acceptable salt thereof, wherein q is 1.

36. A commerical product packaged for treatment of cancer, inflammation, septic shock, premature labor, infertility, pian or an ischemia related condition, wherein said commercial product comprises the commposition of claim 26 packaged together with instruction for said treatment.

37. A compound of claim 2 , or pharmaceutically acceptable salt thereof, wherein said compound is N-[1-(4-Cyano-phenoxymethyl)-2-imidazol-1-yl-ethyl]-4-iodo-benzamide.

Assignments (3)
CHANGE OF NAME Recorded Dec 3, 2009
From: LABORATOIRES SERONO SA
To: MERCK SERONO SA
Reel/Frame 023599/0944 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 11, 2007
From: APPLIED RESEARCH SYSTEMS ARS HOLDING N.V.
To: LABORATOIRES SERONO SA
Reel/Frame 019966/0026 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 18, 2004
From: ARKINSTALL, STEPHEN J.; ARULANANDAM, ANTONIO; JIANG, XULIANG; MAGAR, SHARAD; NABIOULLIN, ROUSTEM; ZHANG, JOHN YINGSHENG; BLUME-JENSEN, PETER
To: APPLIED RESEARCH SYSTEMS ARS HOLDING N.V.
Reel/Frame 015998/0884 →