Vaccine against infectious disease
The present invention relates to the use of a protein termed 64p in the production of vaccines for protecting animals against the bite of blood-sucking ectoparasites and against the transmission of viruses, bacteria and other pathogens by such ectoparasites.
1 . A vaccine composition effective against an infectious disease borne by a blood-feeding ectoparasite, said vaccine comprising as an active component a 64p protein comprising the sequence presented in FIG. 1 , a fragment thereof or a homologue of said 64p protein or protein fragment that exhibits at least 50% sequence identity with said protein or protein fragment.
2 . A composition according to claim 1 , wherein said protein, fragment or homologue contains an immunogenic epitope that is present in a gut protein of a blood-feeding ectoparasite.
3 . A composition according to claim 1 , wherein said infectious disease agent borne by the blood-feeding ectoparasite is the cause of Malaria; Dengue Fever; Yellow Fever and Arboviral Encephalitides, including Eastern Equine Encephalitis, Japanese Encephalitis, LaCrosse Encephalitis, St. Louis Encephalitis, Western Equine Encephalitis, West Nile Virus Encephalitis; Lymphatic filariasis; plague; schistosomiasis; trypanosomiasis; Leishmaniasis; Onchocerciasis; protozoan, rickettsial and viral disease agents of livestock, including those causing East Coast Fever, babesioses, anaplasmoses, cowdriosis and tick-associated dermatophilosis; and the human diseases Lyme Disease; Southern Tick-Associated Rash Illness (STARI); Babesiosis; Ehrlichiosis; Rocky Mountain SpottedFever; tularemia; tick-borne relapsing fever, tick-borne encephalitis and Crimean-Congo Haemorrhagic Fever.
4 . A composition according to claim 1 , wherein said vaccine is effective against the transmission of agents causing disease in mammals (particularly humans and livestock), birds, reptiles and fish.
5 . A composition to claim 1 , wherein said 64p fragment or homologue is derived from a blood-feeding ectoparasite.
6 . A composition according to claim 5 , wherein said blood-feeding ectoparasite is a mosquito, horsefly, sandfly, blackfly, tsetse fly, flea, lice, mite, leech, flatworm or tick.
7 . A composition according to claim 6 , wherein said blood-feeding ectoparasite is a tick.
8 . A composition according to claim 1 , wherein said fragment is any one of 64trp1, 64trp2, 64trp3, 64trp4, 64trp5 or 64trp6 identified herein, or a homologue thereof.
9 . A composition according to claim 8 , wherein said fragment comprises residues 103-132 inclusive of the sequence presented in FIG. 1 (64trp1), or is a homologue thereof.
10 . A composition according to claim 8 , wherein said fragment comprises residues 1-51 inclusive of the sequence presented in FIG. 1 (64trp2), or is a homologue thereof.
11 . A composition according to claim 8 , wherein said fragment comprises residues 1-70 inclusive of the sequence presented in FIG. 1 (64trp3), or is a homologue thereof.
12 . A composition according to claim 8 , wherein said fragment comprises residues 69-132 inclusive of the sequence presented in FIG. 1 (64trp4), or is a homologue thereof.
13 . A composition according to claim 8 , wherein said fragment comprises residues 1-133 inclusive of the sequence presented in FIG. 1 (64trp5), or is a homologue thereof.
14 . A composition according to claim 1 that is effective against both adult and immature forms of the blood-feeding ectoparasite and wherein the blood-feeding ectoparasite is a tick.
15 . A composition according to claim 1 , wherein said 64p protein, fragment or homologue is expressed in recombinant form.
16 . A composition according to claim 1 , additionally comprising a second active agent.
17 . A composition according to claim 16 , wherein said second active agent is a second immunogenic protein, or protein fragment derived from a blood-feeding ectoparasite.
18 . A composition according to claim 17 , wherein said second immunogenic protein, fragment or homologue is a 64p protein or protein fragment.
19 . A composition according to claim 18 , comprising a combination of 64trp2 and 64trp5; a combination of 64trp5 and 64trp6; or a combination of 64trp2 and 64trp6.
20 . A composition according to claim 16 , wherein said active agent is a vaccine against an infectious disease.
21 . A composition according to claim 20 , wherein said vaccine against an infectious disease is a TBE vaccine.
22 . A composition according to claim 1 , additionally comprising an adjuvant.
23 . An antibody or an antiserum that is reactive with a 64p protein, fragment or homologue.
24 . An antibody or antiserum according to claim 23 , wherein said 64p protein, fragment or homologue is selected from the group consisting of the 64p protein comprising the sequence set forth in FIG. 1 , proteins, fragments, and homologues thereof.
25 . A method of production of an antibody or an antiserum, comprising immunising an animal with a vaccine composition in accordance with claim 1 .
26 . A method of immunising an animal against a blood-feeding ectoparasite and/or transmission of an infectious disease caused by a blood-feeding ectoparasite comprising administering to said animal a vaccine composition in accordance with claim 1 .
27 . A 64p protein, fragment thereof or homologue of said 64p protein or protein fragment, for use in a vaccine.
28 . (canceled)