Micro fluidic structures
View Patent ↗The invention relates to a micro fluidic system comprising a substrate, and, provided on said substrate, at least one flow path interconnecting with functional means in which liquid samples can be treated by desired procedures. The flow paths are laid out to form a pattern for the transport of liquid samples to and from said functional means. These flow paths comprise a plurality of micro posts protruding upwards from said substrate, the spacing between the micro posts being small enough to induce a capillary action in a liquid sample applied anywhere within any of said flow paths, so as to force said liquid to move from where said liquid sample was applied.
1. An open micro fluidic system comprising:
a substrate having a non-porous surface;
a first plurality of microposts protruding from said non-porous surface of said substrate and defining at least one flow path in which liquid samples can be treated by desired procedures, wherein said at least one flow path is laid out for transport of liquid samples, wherein the at least one flow path extends in a lateral direction which is transverse to the protruding microposts; wherein cross sections of said microposts and the center to center spacing between each of the first plurality of microposts spontaneously induces a passive capillary action in a liquid sample applied to said at least one flow path, so as to force said liquid to move laterally away from where said liquid sample was applied, said first plurality of microposts further including at least one biological binder having an affinity to at least one component of a liquid sample introduced along said at least one flow path;
a sample receiving area disposed prior to said at least one flow path and fluidly connected therewith; and
a sample collecting sink disposed after said at least one flow path on said substrate and fluidly connected with said at least one flow path, said sample collecting sink having an area which is larger than the area of the at least one flow path extending from said sample receiving area, said sink having a second plurality of microposts protruding from said substrate surface, said second plurality of microposts having cross sections and center to center spacing between each of said microposts that further induce capillary flow from said flow path to said sample collecting sink and wherein of the cross sections and center to center spacing of said second plurality of microposts are different those than of said first plurality of microposts.
2. The micro fluidic system according to claim 1 , wherein said substrate is provided with grooves having a bottom surface and side walls, said first plurality of microposts protruding from the bottom surface of said grooves and wherein the passive capillary action is caused essentially between said liquid and the microposts.
3. The micro fluidic system according to claim 2 , wherein each plurality of microposts has a height dimension, wherein said at least one flow path is covered by a lid, and wherein said lid is positioned at a distance from the surface of the substrate to the lid that is considerably larger than the height dimension of each plurality of microposts so that no capillary action between the substrate and the lid can arise.
4. The micro fluidic system according to claim 3 , wherein the lid has access openings for enabling introduction of at least one of reagents, gas, liquids, and samples into said at least one flow path.
5. The micro fluidic system according to claim 1 , wherein the substrate is essentially flat and each plurality of microposts protrude from said substrate.
6. The micro fluidic system of claim 1 , wherein the first plurality of microposts are grouped in adjacent segments so as to provide a capillary barrier discontinuity between such segments, the capillary barrier discontinuity having a finite distance, preventing the capillary flow between said segments, thereby providing a flow stop.
7. The micro fluidic system according to claim 6 , further comprising means for applying energy to one or more selected segments to induce a forced transport across the flow stop.
8. The micro fluidic system according to claim 7 , wherein said means for applying energy is selected from a pressure pulse generator, an ultrasound generator, and an electromagnetic radiation means.
9. The micro fluidic system according to claim 6 , further comprising means for applying liquid to said discontinuity to provide a bridge across said discontinuity so as to induce a flow there across.
10. The micro fluidic system according to claim 1 , wherein surfaces of the microposts in said at least one flow path have at least one of a chemical, biologic or physical functionality.
11. The micro fluidic system according to claim 10 , wherein the first plurality of microposts have chemically reactive groups on their surface.
12. The micro fluidic system according to claim 10 , wherein the first plurality of microposts have hydrophilic groups on their surfaces.
13. The micro fluidic system according to claim 10 , wherein the first plurality of microposts have positively and/or negatively charged groups on their surfaces.
14. The micro fluidic system according to claim 11 , wherein the first plurality of microposts have hydrophobic structures on their surfaces.
15. The micro fluidic system according to claim 10 , wherein said at least one functionality is applied to the first plurality of microposts in the entire at least one flow path or limited to a portion thereof.
16. The micro fluidic system according to claim 11 , wherein said first plurality of microposts have a property that is selected from the group consisting of a diameter of 20 μm, a height of 100 μm, a square shape, a circular cross section, a hydrophobic surface coating, a hydrophilic surface coating, and a micropost spacing distance from center-to center of 30 μm.
17. The micro fluidic system of claim 1 , wherein particles are provided within said at least one flow path.
18. The micro fluidic system according to claim 17 , wherein said particles are chemically or physically bound to the substrate, or mechanically trapped within a region comprising at least a portion of said first plurality of microposts.
19. The micro fluidic system of claim 1 , wherein said at least one flow path has integrated zones or delimited surfaces containing electrodes or other means for electrical manipulation of liquids and/or reagents.
20. The micro fluidic system of claim 1 , wherein said at least one flow path has integrated zones or delimited surfaces containing optical elements or other means for transmitting, focusing, reflecting or absorbing light.
21. The micro fluidic system of claim 1 , wherein said at least one flow path has integrated zones or delimited surfaces containing means for the regulation of the temperature in said zone.
22. The structure according to claim 1 , wherein said first plurality of micro posts are defined by an aspect ratio of at least 1:2.
23. The structure according to claim 1 , wherein said first plurality of micro posts are defined by an aspect ratio of at least 1:5.
24. The structure according to claim 1 , wherein said first plurality of micro posts are defined by a diameter of approximately 20 micrometers, a height of approximately 100 micrometers and a center to center distance between adjacent micro posts of approximately 30 micrometers.