Methods of increasing protein expression levels
View Patent ↗The present invention relates to methods of increasing protein expression levels whereby at least one amino acid in a protein amino acid sequence is substituted for the amino acid, proline. Preferably, the substitution occurs within 15 amino acids, more preferably within 10 amino acids and most preferably within 5 amino acids of a cysteine amino acid residue. The present invention not only includes methods for polypeptides with proline substitutions, but also polynucleotides with codon substitutions for which a codon for any amino acid, except proline, is substituted for a codon encoding for proline.
1. An isolated mutant of a native polypeptide wherein the native polypeptide comprises the amino acid sequence of SEQ ID NO: 1, and wherein the amino acid at position 23 or the amino acid at position 76 of SEQ ID NO: 1 is substituted with proline and wherein said mutant exhibits increased expression compared with that of the native polypeptide.
2. The polypeptide of claim 1 , wherein the amino acid at position 23 of SEQ ID NO: 1 is substituted with proline.
3. The polypeptide of claim 1 , wherein the amino acid at position 76 of SEQ ID NO: 1 is substituted with proline.
4. A method of producing an isolated mutant of a native polypeptide comprising:
a. providing an in vitro host cell comprising an isolated nucleic acid encoding the polypeptide of claim 1 , 2 or 3 ; and
b. expressing the mutant polypeptide; and optionally
c. recovering the mutant polypeptide.
5. A method of producing an isolated mutant of a native polypeptide comprising:
a. providing an in vitro host cell comprising an expression vector comprising an isolated nucleic acid encoding the polypeptide of claim 1 , 2 or 3 ; and
b. expressing the mutant polypeptide; and optionally
c. recovering the mutant polypeptide.