Micro-band electrode
View Patent ↗The invention concerns an electrochemical cell which, either alone or together which a substrate onto which it is placed, is in the form of a receptacle. The electrochemical cell contains a working electrode and a counter electrode, the working electrode being in a wall of the receptacle. At least one of the electrodes has at least one dimension of less than 50 μm. The electrochemical cell is principally intended for use as a micro-electrode suitable for screening water, blood, urine or other biological or non-biological fluids.
1. An electrochemical cell in the form of a receptacle, said cell comprising a counter electrode and a working electrode, wherein the minimum distance between the working electrode and the counter electrode is 50 μm, wherein at least one electrode is a micro-electrode having one dimension of less than 50 μm and one dimension of greater than 50 μm, wherein the working electrode is in a wall of the receptacle, and wherein the receptacle contains an electro-active substance, and wherein the receptacle is shaped so as to restrict movement of the electro-active substance away from the electrodes when a sample flows over the electrochemical cell.
2. An electrochemical cell according to claim 1 , wherein the working electrode is in the form of a continuous band.
3. An electrochemical cell according to claim 1 , which further comprises a reference electrode.
4. An electrochemical cell according to claim 1 , wherein the ratio of the surface area of the counter electrode to the surface area of the working electrode is at least 25:1.
5. An electrochemical cell according to claim 1 , wherein the working electrode is a micro-electrode having at least one dimension in the range of 0.1 to 20 μm.
6. An electrochemical cell according to claim 1 , wherein the receptacle has a width, or in the case of a cylindrical receptacle a diameter, of from 0.1 to 5 mm.
7. An electrochemical cell according to claim 1 , wherein the minimum distance between the counter electrode and the working electrode is from 50 to 1000 μm.
8. An electrochemical cell according to claim 1 , wherein a base and/or a wall or walls of the receptacle contain one or more air-outlet channels.
9. An electrochemical cell according to claim 1 , wherein the electro-active substance has been dried.
10. An electrochemical cell according to claim 1 , wherein the electro-active substance comprises an enzyme.
11. An electrochemical cell according to claim 1 , wherein the base of the receptacle comprises an electro-active substance.
12. An electrochemical cell according to claim 1 , wherein the base of the receptacle comprises a membrane, said membrane comprising an electro-active substance.
13. An electrochemical cell according to claim 1 , wherein the open end of the receptacle is at least partly covered by a permeable membrane.
14. An electrochemical cell according to claim 13 wherein the permeable membrane comprises an electro-active substance.
15. An electrochemical cell according to claim 1 , wherein the receptacle comprises one or more capillary flow channels through which a sample may enter.
16. An electrochemical cell according to claim 1 , wherein the counter electrode forms at least a part of a base of the receptacle.
17. An electrochemical cell according to claim 15 , wherein the receptacle is covered by a layer containing the counter electrode.
18. An electrochemical cell according to claim 1 , wherein the counter electrode is in a wall or walls of the receptacle.
19. An electrochemical cell according to claim 1 which is suitable for screening liquid samples.
20. A multi-analyte device comprising a plurality of electrochemical cells according to claim 1 .
21. A process for producing an electrochemical cell according to claim 1 , which process comprises the steps of:
(a) forming a first part comprising an insulating material which is optionally coated with a counter electrode layer;
(b) forming a second part comprising a laminate of a working electrode layer between two layers of an insulating material;
(c) creating a hole in the second part; and
(d) bonding said first part to said second part to form a receptacle, which process further comprises placing an electro-active substance into the receptacle and optionally drying the electro-active substance, and wherein the receptacle is shaped so as to restrict movement of the electro-active substance away from the electrodes when a sample flows over the electrochemical cell.
22. A process according to claim 21 wherein the second part comprises a laminate of a counter electrode layer between two layers of insulating material.
23. A process according to claim 21 wherein the first part comprises an electro-active substance.
24. A process according to claim 21 , which further comprises placing a membrane over at least a part of the open end of the receptacle.
25. A process according to claim 21 , wherein step (c) comprises forming two or more holes in said second part, in order to form a multi-analyte device.
26. A process according to claim 21 which comprises the steps of:
(a) forming a first part comprising an insulating material;
(b) forming a second part comprising a laminate of a working electrode layer between two layers of an insulating material;
(c) creating, in the second part, a hole and a capillary channel to allow a sample to enter said hole;
(d) bonding said first part to said second part to form a receptacle;
(e) placing an electro-active substance into the receptacle and optionally drying the electro-active substance; and
(f) bonding to the open end of said receptacle a layer which is optionally coated with a counter electrode material.
27. A process according to claim 26 , wherein step (c) comprises forming, in said second part, two or more holes and two or more capillary channels to allow a sample to enter said two or more holes, and wherein step (e) comprises inserting an electro-active substance, which may be identical or different, into one or more of the receptacles formed in step (d), in order to form a multi-analyte device.
28. A process according to claim 21 , wherein one or more of the electrodes is formed by screen or inkjet printing onto a substrate.
29. A method of electrochemically testing one or more compounds of a sample, the method comprising the steps of:
(a) providing an electrochemical cell in the form of a receptacle, said cell comprising a counter electrode and a working electrode, wherein the minimum distance between the working electrode and the counter electrode is 50 μm, wherein at least one electrode is a micro-electrode having one dimension of less than 50 μm and one dimension of greater than 50 μm, wherein the working electrode is in a wall of the receptacle, wherein the receptacle contains an electro-active substance, and wherein the receptacle is shaped so as to restrict movement of the electro-active substance away from the electrodes when a sample flows over the electrochemical cell;
(b) inserting the sample into the electrochemical cell;
(c) applying a voltage or a current between the working and counter electrodes; and
(d) measuring the resulting current, voltage or charge across the working and counter electrodes.
30. A multi-analyte device comprising a plurality of electrochemical cells as defined in claim 13 .
31. An electrochemical cell according to claim 1 , wherein the ratio of the surface area of the counter electrode to the surface area of the working electrode is about 1:1.
32. An electrochemical cell according to claim 1 , wherein the ratio of the surface area of the counter electrode to the surface area of the working electrode is from 1:1 to 25:1.
33. An electrochemical cell according to claim 1 , wherein the working electrode is a micro-electrode having at least one dimension of less than 25 μm.
34. An electrochemical cell according to claim 1 , wherein the receptacle has a width, or in the case of a cylindrical receptacle a diameter, of at least about 1 mm.
35. A process according to claim 21 , wherein the step of creating a hole in the second part comprises a laser drilling step.