Nucleic acids encoding novel cyclic GMP dependent protein kinases from
The present invention relates to novel protozoal cyclic GMP dependent protein kinases and genes encoding same that have been isolated from Eimeria maxima and Plasmodium falciparum . Nucleic acid molecules having sequences that encode such proteins as well as antibodies raised against such proteins are also disclosed. These enzymes may be used in screening assays to identify potential antiprotozoal agents. As well, methods to obtain such nucleic acid molecules, proteins, antibodies and inhibitor are also provided as are therapeutic compositions comprising such nucleic acid molecules, proteins, antibodies and inhibitors as well as their use to protect animals from disease caused by parasites such as Eimeria maxima and Plasmodium falciparum.
1. An isolated nucleic acid molecule, comprising a sequence of nucleotides that encode an Eimeria maxima cGMP dependent protein kinase (PKG), wherein the sequence of nucleotides is selected from the group consisting of:
(a) a sequence of nucleotides that encodes an Eimeria maxima cGMP dependent protein kinase (PKG) comprising the nucleotide sequence of SEQ ID NO: 1; and
(b) a sequence of nucleotides that encodes an Eimeria maxima cGMP dependent protein kinase (PKG) comprising the amino acid sequence of SEQ ID NO:2.
2. A recombinant nucleic acid molecule comprising a nucleic acid sequence according to claim 1 operatively linked to expression control sequences enabling expression of said nucleic acid sequence.
3. An isolated host cell transformed with the recombinant nucleic acid molecule according to claim 2 .
4. An immunogenic composition, comprising a recombinant nucleic acid molecule according to claim 2 , together with a pharmaceutically acceptable carrier.
5. An immunogenic composition, comprising the nucleic acid molecule of claim 1 , together with a pharmaceutically acceptable carrier.
6. An isolated nucleic acid molecule that encodes an Eimeria maxima cGMP dependent protein kinase (PKG) comprising the amino acid sequence of SEQ ID NO:2.
7. The isolated nucleic acid molecule of claim 6 wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID NO: 1.