Separation method
View Patent ↗The present invention relates to a method of separating a compound from a liquid, which method comprises providing a separation matrix comprising at least one uncharged ligand; providing a liquid wherein the compound to be separated is present in a positively charged state; contacting said matrix with said liquid to adsorb the compound; and removing the liquid. The uncharged ligands possess a quadrupole or dipole moment, allowing for a cation-π interaction between the compound and the ligand. The present invention also encompasses the use of a separation matrix, which comprises an uncharged group that possesses a quadrupole or dipole moment, in said method.
1. A method of separating one or more proteins from an aqueous solution, which method comprises the steps of:
(a) providing a separation matrix including a C═C double bond or a C≡N triple bond or an aromatic group as at least one uncharged ligand;
(b) providing an aqueous solution wherein each protein to be separated is present in a positively charged state;
(c) contacting, under a low ionic strength of less than 0.2 M, said matrix with said aqueous solution for a sufficient period of time to permit adsorption of each protein to the ligand to occur while maintaining each of said protein in a positively charged state;
(d) removing the aqueous solution from the matrix; and
(e) eluting at least one protein from the separation matrix;
wherein each protein to be separated includes one or more basic amino acids, wherein the adsorption of each protein to said ligand is predominated by cation-π interaction and wherein the adsorption during step (c) is performed at a pH<pl, and the desorption during step (e) is performed at a pH>pl.
2. The method of claim 1 , wherein the ligand is coupled to a support via a spacer, which spacer distances the ligand from the support.
3. The method of claim 2 , wherein said spacer includes one or more functionalities capable of interacting with the protein to be separated.
4. The method of claim 1 , wherein the elution is performed by adding an organic solution.
5. The method of claim 1 , wherein said low ionic strength is about 0.1 M.