Monomers useful in the preparation of separation matrices
View Patent ↗The invention relates to an acrylic monomer defined by formula (I): wherein R 1 , R 2 and R 3 are H or CH 3 ; R 4 and R 5 are H or OH; R 6 and R 7 are H or OH; R 8 is H or CH 3 ; m is 2–4; and n and p are 0 or 1. The invention also relates to a polymer comprised of repeated such monomer units as well as a separation matrix prepared thereof.
1. A cross-linked aqueous matrix comprising a water-insoluble copolymer comprising repeating units of an acrylic monomer suitable for use in electrophoresis, comprising the general formula
wherein
R 1 , R 2 and R 3 are each H or CH 3 , respectively;
R 4 and R 5 are each H or OH, respectively;
R 6 and R 7 , if present, are each H or OH, respectively, with the proviso that at least two of R 4 , R 5 , R 6 and R 7 are OH;
R 8 is H or CH 3 , respectively;
m is an integer of 2–4; and
n and p are each integers of 0 or 1 with the proviso that if n is 0, then R 6 is not present, and if p is 0, the R 7 is not present;
said matrix further comprising a mixture of the monomer defined by the general formula (II)
—(NH) t C(═NH)(NH 2 ) (II)
wherein t is an integer of 0 or 1,
together with other monomers containing acidic and basic groups to determine the pH resulting within the matrix.
2. The matrix of claim 1 , wherein the monomer defined by formula (II) is agmatine.
3. The matrix of claim 1 , further comprising functionalizing pH-buffering groups.
4. The matrix of claim 3 , wherein said pH-buffering groups exhibit pKa values of above about 9.
5. The matrix of claim 1 , which includes a pH gradient of from about 6–12.
6. The matrix of claim 1 , which has been polymerised in a mold for use in electrophoretic separation.
7. The matrix of claim 1 , which is in the form of a sheet, a rod or a thin filament.
8. The matrix of claim 1 , which has been grafted onto a solid support, such as a plastic or glass support.
9. The matrix of claim 1 , wherein the repeating units have been cross-linked with bis-acrylamide.