Novel promoter
Disclosing a promoter of the protease MDTS9, which is induced by TGF-β, which is involved in the metabolism of extracellular matrix, and in which the expression of the gene thereof is elevated in renal failure model animals, which promoter is useful as a tool for screening an agent for treating and/or preventing chronic renal failure, as well as a method for screening an agent for treating and/or preventing chronic renal failure by selecting an inhibitor of the promoter.
1 . An isolated polynucleotide molecule having a promoter activity for a polynucleotide encoding the amino acid sequence of SEQ ID NO:2, wherein said polynucleotide molecule comprises a polynucleotide sequence selected from the group) consisting of:
(a) the polynucleotide sequence of SEQ ID NO:17,
(b) a polynucleotide sequence of SEQ ID NO:17 in which 1 to 10 nucleotides are substituted, deleted, and/or inserted,
(c) the polynucleotide sequence consisting of nucleotides 3253 to 5023 of SEQ ID NO:17, and
(d) a polynucleotide sequence consisting of nucleotides 3253 to 5023 of SEQ ID NO:17 in which 1 to 10 nucleotides are substituted, deleted, and/or inserted.
2 . The polynucleotide molecule according to claim 1 , wherein said polynucleotide molecule comprises the polynucleotide sequence of SEQ ID NO:17 or a polynucleotide sequence of SEQ ID NO:17 in which 1 to 10 nucleotides are substituted, deleted, and/or inserted.
3 . The polynucleotide molecule according to claim 1 , wherein said polynucleotide molecule comprises the polynucleotide sequence consisting of nucleotides 3253 to 5023 of SEQ ID NO:17 or the polynucleotide sequence of SEQ ID NO:17.
4 . An expression vector comprising the polynucleotide molecule according to any one of claims 1 to 3 .
5 . A cell transfected with the expression vector according to claim 4 .
6 - 7 . (canceled)
8 . A method for analyzing a test compound about whether or not the test compound inhibits the promoter activity of the polynucleotide according to any one of claims 1 to 3 , comprising (1) a step of allowing the test compound to contact with the cell according to claim 5 and (2) a step of detecting the promoter activity.
9 . A method for screening a substance suppressing the expression of a polypeptide of SEQ ID NO:2, comprising the analyzing step by the method according to claim 8 and a step of selecting a substance inhibiting the promoter activity.
10 . (canceled)
11 . A method for producing a pharmaceutical composition for the treatment and/or prevention of chronic renal failure, comprising the analyzing step by the method according to claim 8 and a formulation step.
12 . The polynucleotide molecule according to claim 1 , wherein said polynucleotide molecule consists of the polynucleotide sequence of nucleotides 3253 to 5023 of SEQ ID NO:17 or the polynucleotide sequence of SEQ ID NO:17.