Polynucleotide encoding a 35 KDA protein thats binds to WF00144
View Patent ↗It is intended to provide a protein participating in the regulation of sugar production, a polynucleotide encoding the same, a method of screening a compound participating in the regulation of sugar production and a drug for treating or preventing diabetes which contains the compound participating in the regulation of sugar production. A polynucleotide encoding a protein specifically binding to a substance WF00144 is found out from rat liver cells and thus a protein participating in the regulation of sugar production and a polynucleotide encoding the same are provided. Moreover, a method and a substance relating to a method of treating and preventing diabetes are found out and thus a method of treating diabetes relating to the regulation of sugar production relating to the method of treating and preventing diabetes is provided.
1. An isolated polynucleotide:
which consists of a coding region for a polypeptide, wherein said polypeptide binds to WF00144 and has a molecular weight of about 35 kD; and
said polynucleotide has at least 96% sequence identity to SEQ ID NO: 1, or which hybridizes to the complement of SEQ ID NO: 1 under stringent conditions, wherein stringent conditions comprise washing in 0.1×SSC and 0.1% SDS at 68° C.
2. The isolated polynucleotide of claim 1 , which has at least 96% sequence identity to SEQ ID NO: 1.
3. The isolated polynucleotide of claim 1 , which hybridizes to the complement of SEQ ID NO: 1 under stringent conditions, wherein stringent conditions comprising washing in 0.1×SSC and 0.1% SDS at 68° C.
4. The isolated polynucleotide of claim 1 , which encodes a polypeptide comprising SEQ ID NO: 2.
5. The isolated polynucleotide of claim 1 , which consists of SEQ ID NO: 1.
6. The isolated polynucleotide of claim 1 , wherein said encoded polypeptide has at least 96% sequence identity to SEQ ID NO: 2.
7. The isolated polynucleotide of claim 1 , which encodes a polypeptide in which one to five of the amino acid residues in SEQ ID NO: 2 are different than the corresponding amino acids in SEQ ID NO: 2.
8. An expression vector comprising the polynucleotide of claim 1 , which expresses a polypeptide that binds to WF00144 and has a molecular weight of about 35 kDa.
9. A transformant comprising the polynucleotide of claim 1 .
10. The full complement of the polynucleotide of claim 1 .
11. The isolated polynucleotide of claim 1 , further consisting of at its 5′ end an oligonucleotide encoding histidine, lysine or arginine.
12. A viral vector comprising the isolated polynucleotide of claim 1 .
13. A liposome comprising the isolated polynucleotide of claim 1 .
14. A method for making a polypeptide that binds to WF00144, comprising culturing the transformant of claim 9 under conditions suitable for production of a polypeptide that binds to WF00144, and recovering said polypeptide.