Generation of plants with improved pathogen resistance
View Patent ↗The present invention is directed to plants that display a pathogen resistance phenotype due to altered expression of a PPR2 nucleic acid. The invention is further directed to methods of generating plants with a pathogen resistance phenotype.
1. A method of producing a plant having increased pathogen resistance, said method comprising:
a) introducing into cells of the plant a plant transformation vector comprising a nucleotide sequence that encodes a Myb polypeptide that confers Peronospora parasitica resistance (PPR2), said Myb polypeptide selected from the group consisting of: the polypeptide sequence of SEQ ID NO:2; a polypeptide sequence having at least 95% sequence identity to SEQ ID NO:2; and an Arabidopsis thaliana polypeptide having at least 85% sequence identity to SEQ ID NO:2, and
b) growing the transformed cells to produce a transgenic plant, wherein said nucleotide sequence is expressed, and said transgenic plant exhibits increased pathogen resistance relative to a control plant.
2. A transformed plant obtained by the method of claim 1 .
3. A transformed plant part obtained from the plant according to claim 2 .
4. The transformed plant of claim 2 , wherein the nucleotide sequence is set forth in SEQ ID NO: 1.
5. The transformed plant of claim 2 , wherein the nucleotide sequence encodes an Arabidopsis thaliana Myb polypeptide having at least 85% sequence identity to SEQ ID NO: 2.
6. The transformed plant of claim 5 , wherein the Arabidopsis thaliana Myb polypeptide has at least 95% sequence identity to SEQ ID NO:2.
7. A method of producing a plant having increased pathogen resistance, said method comprising:
a) introducing into cells of the plant a plant transformation vector comprising a nucleotide sequence that encodes or is complementary to a sequence that encodes Peronospora parasitica resistance polypeptide comprising the amino acid sequence of SEQ ID NO: 2, SEQ ID NO: 11, or SEQ ID NO: 12; and
b) growing the transformed cells to produce a transgenic plant, wherein said nucleotide sequence is expressed, and said transgenic plant exhibits increased pathogen resistance relative to a control plant.
8. A transformed plant produced by the method of claim 7 .
9. The transformed plant of claim 8 , wherein the nucleotide sequence encodes SEQ ID NO: 2.
10. The transformed plant of claim 9 , wherein the nucleotide sequence comprises SEQ ID NO: 1.
11. The method of claim 1 , wherein the transformation vector further comprises a constitutive promoter operably linked to the nucleotide sequence.
12. The method of claim 1 , wherein the transformation vector further comprises a pathogen inducible promoter operably linked to the nucleotide sequence.
13. The transformed plant of claim 5 , wherein the nucleotide sequence encodes an Arabidopsis thaliana Myb polypeptide having at least 90% sequence identity to SEQ ID NO: 2.