Membraine filtration module and method for the separation of biomolecules from a liquid
The invention describes a membrane and a method of making and using the same, the membrane being made from a microporous membrane body with an affinity ligand capable of interacting with at least one type of biomolecule found in a fluid, whereby the membrane body may be stored in a dried state yet retain the activity of the affinity ligand.
1 . A method of preparing a membrane having an affinity for biomolecules comprising the steps:
(a) providing a microporous membrane;
(b) reacting said membrane of step (a) with a reagent containing a functional group to form a functionalized membrane containing reactive functional groups on the surfaces thereof;
(c) contacting said functionalized membrane of step (b) with a solution containing an affinity ligand to couple said ligand to said functional group to form a biologically active membrane;
(d) washing said biologically active membrane with a washing solution containing a volatile organic compound that is miscible with said washing solution; and
(e) drying said biologically active membrane.
2 . The method of claim 1 wherein said functional group of step (b) is an aldehyde.
3 . The method of claim 1 wherein said affinity ligand of step (c) is selected from the group consisting of thiophiles; hydrophobes; reversed phase ligands; dyes; low molecular weight charged or non-charged organic molecules; amino acids and analogs thereof; coenzymes, cofactors and analogs thereof; substrates and analogs thereof; endocrine and exocrine substances; enzyme substrates, enzyme inhibitors and analogs thereof; fatty acids, fatty acid derivatives, conjugated fatty acids and analogs thereof; nucleic acids; monomers and analogs and derivatives thereof; polymers and oligopolymers and analogs and derivatives thereof; high molecular weight carbohydrates; glycolic conjugates; proteins and oligomers, subunits and parts thereof; peptides, polypeptides and analogs and derivatives thereof; lectine; antibodies and parts thereof; fusion proteins; haptenes; enzymes and subunits and parts thereof; structural proteins; receptors and parts thereof; xenobiotics; pharmaceuticals and pharmaceutically active substances; alkaloids; antibiotics; and biomimmicking substances.
4 . The method of claim 3 wherein said affinity ligand is Protein A.
5 . The method of claim 1 wherein said washing solution of step (d) is aqueous-based.
6 . The method of claim 5 wherein said washing solution is a phosphate buffered saline solution.
7 . The method of claim 6 wherein said volatile organic compound of step (c) is glycerine.
8 . The method of claim 1 wherein step (c) is conducted at ambient temperature.
9 . The method of claim 1 wherein said microporous membrane of step (a) is a polymeric membrane selected from the group consisting of cellulose acetate, cellulose nitrate, polyamide, polyethersulfone, polypropylene and polyvinylidene fluoride.
10 . The method of claim 9 wherein said microporous membrane has an average pore diameter of from about 0.01 to about 15 microns and a thickness of from about 100 to about 500 microns.
11 . The membrane product of the method of any of claims 1 - 4 .
12 . The membrane product of claim 11 stored in a dry state in a substantially anaerobic atmosphere.
13 . At least one of the membrane product of claim 11 in a filtration housing having a fluid inlet and a fluid outlet wherein said at least one membrane product is situated between said inlet and said outlet.
14 . The membrane product of claim 13 wherein said filtration housing contains three of said membrane products.