Chemiluminescence enhancer

1. A method of producing chemiluminescence in a solid phase immunoassay, comprising:

contacting at least one antigen or/and an antibody immobilized onto fine solid carriers dispersed in a liquid medium with a chemiluminescent substrate comprising:

at least one dioxetane,

an enzyme for performing chemiluminescence, and

at least one isolated pretreated chemiluminescence enhancer selected from the group consisting of a water soluble quaternary ammonium salt, a water soluble sulfonium salt and a water soluble quaternary phosphonium salt, each of which has been pretreated with an oxidizing agent or a reducing agent and then isolated from the oxidizing or reducing agent, wherein said isolated pretreated chemiluminescence enhancer enhances the emission of light caused by the reaction of said chemiluminescent substrate with the said enzyme.

2. The method according to claim 1 , wherein the chemiluminescence enhancer does not substantially comprise a component with a molecular weight of more than 400,000 daltons in molecular weight as separated by an ultrafiltration method.

3. The method according to claim 1 , wherein the chemiluminescent substrate comprises at least one dioxetane represented by general formula:

wherein R 2 is an aryl group substituted with an X-oxy group, which forms 1,2-dioxetane compound which is an unstable oxide intermediate when X is eliminated by activator enzyme to induce a reaction, which unstable 1,2-dioxetane compound is decomposed with releasing electron energy to produce light and two carbonyl-containing compounds of general formulae,

and X is a chemically easily reactive group which is eliminated by an enzyme;

R 1 is one selected from the group consisting of an alkyl group, an alkoxy group, an aryloxy group, a dialkylamino group, a trialkylsilyloxy group, an arylsilyloxy group, an aryl group and an aryl group which is bound to an aryl group R 2 to form a polycyclic aryl group with X-oxy group substitution, which spiro-binds to a 1,2-dioxetane ring;

R 3 and R 4 are each an alkyl group or a heteroalkyl group, or R 3 and R 4 may be together bound to form a polycyclic alkylene group which spiro-binds to the 1,2-dioxetane ring.

4. The method according to claim 1 , wherein the chemiluminescence enhancer is prepared from a monomer selected from the group consisting of a quaternary ammonium salt, a sulfonium salt, a quaternary phosphonium salt, and mixtures thereof.

5. The method according to claim 1 , wherein the chemiluminescence enhancer is a polymerized quaternary ammonium salt, a polymerized sulfonium salt, a polymerized quaternary phosphonium salt, or copolymers thereof.

6. The method according to claim 1 , wherein the chemiluminescence enhancer is selected from the group consisting of poly[vinylbenzyl(benzylmethyl ammonium chloride)], poly(vinylbenzyltrimethyl ammonium chloride), poly[vinylbenzyl(tributyl ammonium chloride)], benzylmethylcetyl ammonium chloride, polymethacrylamidepropylenemethyl ammonium chloride, poly[vinylbenzyl(triethyl ammonium chloride)], poly[vinylbenzyl(2-benzylamino)ethyldimethyl ammonium chloride], poly[vinylbenzyldimethyl(2-hydroxy)ethyl ammonium chloride], poly[vinylbenzyl(trimethylphosphonium chloride)], poly[vinylbenzyl(tributylphosphonium chloride and poly[vinylbenzyl(trioctylphosphonium chloride)] and copolymers thereof.

7. The method according to claim 1 , wherein the solid carrier is a particle.

8. The method according to claim 7 , wherein the particle is a magnetic particle.

9. The method according to claim 1 , wherein the chemiluminescence enhancer has been treated with at least one oxidizing agent or a reducing agent selected from the group consisting of ammonium persulfate, sodium sulfite, sodium hypochiorite, hydrogen peroxide, sodium metaperiodate, potassium permanganate and potassium dichromate.

10. The method according to claim 1 , wherein the enzyme is at least one of acid phosphatase, alkali phosphatase, glucosidase, glucuronidase or esterase.

11. The method according to claim 9 , wherein the chemiluminescence enhancer has been treated with at least one oxidizing agent or a reducing agent selected from the group consisting of sodium hypochlorite and sodium metaperiodate.