Vaccine for feline infectious peritonitis
FIP vaccines were provided that use an N protein with a specific structure, or a fragment thereof, as an antigen. Preferred antigens of this invention are N proteins derived from a specific type I virus strain (KU-2). Vaccines comprising such an N protein confer preventive effects against a wide range of FIPVs. In addition, the N proteins are very safe because they do not comprise epitopes that enhance infection. Furthermore, preventive effects can be accomplished against type I viruses, which actually cause 70% or more of FIP.
1 . A vaccine for treating and/or preventing feline infectious peritonitis, wherein said vaccine comprises a protein comprising an amino acid sequence encoded by a polynucleotide of any one of a) to e) as the active ingredient:
a) a polynucleotide comprising a coding region of the nucleotide sequence of SEQ ID NO: 1;
b) a polynucleotide comprising a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO: 2;
c) a polynucleotide comprising a nucleotide sequence with 93% or more homology to a nucleotide sequence of a coding region of the nucleotide sequence of SEQ ID NO: 1;
d) a polynucleotide comprising a nucleotide sequence with 93% or more homology to the nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 2; and
e) a polynucleotide encoding a continuous amino acid sequence comprising 45 or more amino acid residues, selected from an amino acid sequence encoded by the polynucleotide of any one of a) to d).
2 . A vaccine for treating and/or preventing feline infectious peritonitis, wherein said vaccine comprises a polynucleotide of any one of a) to e) as the active ingredient:
a) a polynucleotide comprising a coding region of the nucleotide sequence of SEQ ID NO: 1;
b) a polynucleotide comprising a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO: 2;
c) a polynucleotide comprising a nucleotide sequence with 93% or more homology to a nucleotide sequence of a coding region of the nucleotide sequence of SEQ ID NO: 1;
d) a polynucleotide comprising a nucleotide sequence with 93% or more homology to the nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 2; and
e) a polynucleotide encoding a continuous amino acid sequence comprising 45 or more amino acid residues, selected from an amino acid sequence encoded by the polynucleotide of any one of a) to d).
3 . The vaccine of claim 1 , wherein the polynucleotide is the polynucleotide of a) or b).
4 . An antibody formulation for treating and/or preventing feline infectious peritonitis, wherein said formulation comprises, as an active ingredient, an antibody that can bind to a protein comprising an amino acid sequence encoded by a polynucleotide of any one of a) to e):
a) a polynucleotide comprising a coding region of the nucleotide sequence of SEQ ID NO: 1;
b) a polynucleotide comprising a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO: 2;
c) a polynucleotide comprising a nucleotide sequence with 93% or more homology to a nucleotide sequence of a coding region of the nucleotide sequence of SEQ ID NO: 1;
d) a polynucleotide comprising a nucleotide sequence with 93% or more homology to the nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 2; and
e) a polynucleotide encoding a continuous amino acid sequence comprising 45 or more amino acid residues, selected from an amino acid sequence encoded by the polynucleotide of any one of a) to d).
5 . A method for treating and/or preventing feline infectious peritonitis, wherein said method comprises the process of administering the vaccine of claim 1 to a cat at least once.
6 . A method for treating and/or preventing feline infectious peritonitis, wherein said method comprises the process of administering the antibody formulation of claim 4 to a cat at least once.
7 . A method of testing for feline infectious peritonitis virus infection, wherein said method comprises the steps of:
incubating cat serum with a protein comprising an amino acid sequence encoded by a polynucleotide of any one of a) to e):
a) a polynucleotide comprising a coding region of the nucleotide sequence of SEQ ID NO: 1;
b) a polynucleotide comprising a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO: 2;
c) a polynucleotide comprising a nucleotide sequence with 93% or more homology to a nucleotide sequence of a coding region of the nucleotide sequence of SEQ ID NO: 1;
d) a polynucleotide comprising a nucleotide sequence with 93% or more homology to the nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 2; and
e) a polynucleotide encoding a continuous amino acid sequence comprising 45 or more amino acid residues, selected from an amino acid sequence encoded by the polynucleotide of any one of a) to d); and
detecting an antibody that binds to the protein.
8 . A feline infectious peritonitis viral infection test reagent, comprising a protein that comprises an amino acid sequence encoded by a polynucleotide of any one of a) to e):
a) a polynucleotide comprising a coding region of the nucleotide sequence of SEQ ID NO: 1;
b) a polynucleotide comprising a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO: 2;
c) a polynucleotide comprising a nucleotide sequence with 93% or more homology to a nucleotide sequence of a coding region of the nucleotide sequence of SEQ ID NO: 1;
d) a polynucleotide comprising a nucleotide sequence with 93% or more homology to the nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 2; and
e) a polynucleotide encoding a continuous amino acid sequence comprising 45 or more amino acid residues, selected from an amino acid sequence encoded by the polynucleotide of any one of a) to d).
9 . The vaccine of claim 2 , wherein the polynucleotide is the polynucleotide of a) or b).
10 . A method for treating and/or preventing feline infectious peritonitis, wherein said method comprises the process of administering the vaccine of claim 2 to a cat at least once.
11 . A method for treating and/or preventing feline infectious peritonitis, wherein said method comprises the process of administering the vaccine of claim 3 to a cat at least once.
12 . A method for treating and/or preventing feline infectious peritonitis, wherein said method comprises the process of administering the vaccine of claim 9 to a cat at least once.