Low pressure sperm cell separation system
View Patent ↗A sperm cell process system providing to generate sperm cell insemination samples having controlled sperm cell fertility characteristics of sperm cells.
1. A method of generating an insemination sample comprising sperm cells having at least one modified, controlled fertility characteristic, the method comprising:
a) obtaining a sperm cell sample from a mammal, said sperm cell sample having at least one fertility characteristic;
b) generating a fluid stream within a flow cytometer or cell sorter to provide a fluid stream pressure capable of modifying said at least one fertility characteristic in said sperm cell sample;
c) adjusting said fluid stream pressure to control modification of said at least one fertility characteristic;
d) exposing said sperm cell sample of a) to said fluid stream pressure of c); and
e) collecting said sperm cell sample after said exposing step d),
wherein said collecting of said sperm cell sample provides an insemination sample comprising sperm cells having said at least one modified, controlled fertility characteristic.
2. The method as described in claim 1 , wherein said mammal is selected from the group consisting of: a bovine mammal, an equine mammal, an ovine mammal, a canine mammal, a feline mammal, a porcine mammal, a marine mammal, a cervid mammal, a primate mammal, and a caprid mammal.
3. The method as described in claim 1 , further comprising the step of surrounding said fluid stream with a sheath fluid stream.
4. The method as described in claim 1 , wherein said at least one fertility characteristic is selected from the group consisting of: sperm cell motility, sperm cell viability, oocyte cleavage rate, blastocyst formation rate, pregnancy rate, and a combination thereof.
5. The method as described in claim 3 , wherein said sheath fluid stream includes a sheath fluid selected from the group consisting of: a phosphate buffered saline (PBS), a citrate buffer, a 2.9% sodium citrate buffer, a HEPES buffer, a TRIS-based sheath fluid, and a combination thereof.
6. The method as described in claim 3 , wherein said mammal is a bovine mammal and said insemination sample comprises between about 1×10 5 and about 2×10 7 bovine sperm cells.
7. The method as described in claim 1 , further comprising adjusting the fluid stream pressure of step c) incrementally to modify said at least one fertility characteristic of said sperm cell sample.
8. The method as described in claim 7 , wherein adjusting said fluid stream pressure occurs between about 20 psi (0.098 kg/cm 2 ) and about 100 psi (7.03 kg/cm 2 ).
9. The method as described in claim 8 , wherein said adjusting said fluid stream pressure occurs between about 30 psi (2.10 kg/cm 2 ) and about 40 psi (2.81 kg/cm 2 ).
10. The method as described in claim 9 , wherein said fluid stream pressure is selected from the group consisting of: about 30 psi when sperm cell motility is the at least one fertility characteristic; about 40 psi when sperm cell motility is the at least one fertility characteristic; about 30 psi when sperm cell viability is the at least one fertility characteristic; about 40 psi when sperm cell viability is the at least one fertility characteristic; about 40 psi when oocyte cleavage rate is the at least one fertility characteristic; about 40 psi when blastocyst formation rate is the at least one fertility characteristic; and about 40 psi when pregnancy rate is the at least one fertility characteristic.
11. The method as described in claim 4 , where said mammal is a bovine mammal, said at least one fertility characteristic is pregnancy rate, and pregnancy rate is determined as the number of pregnancies resulting from insemination of a female bovine mammal with said insemination sample comprising sperm cells having at least one modified, controlled fertility characteristic.
12. The method as described in claim 2 , wherein the mammal is a bovine mammal and said insemination sample contains between 1×10 6 and 3×10 6 bovine sperm cells.
13. The method as described in claim 1 , further comprising sorting the sperm cells in said insemination sample by X-chromosome or Y-chromosome.