Human antihuman interleukin-6 antibody and fragment of antibody
View Patent ↗A substance effective for treating immunopathy where interleukin 6 (IL-6) is involved is provided. A human anti-human IL-6 antibody and a human anti-human IL-6 antibody fragment having a high affinity to human IL-6 were obtained using phage antibody technique. The antibody and antibody fragment are expected to be useful as a medicament for treating inflammation and immunopathy caused by IL-6.
1. A gene fragment coding for a single chain Fv (hereinafter referred to as “scFv”) of a human anti-human IL-6 antibody that binds to human IL-6 with a dissociation constant (K D ) of 1.0×10 −8 M or less,
said gene fragment consisting of a gene fragment coding for a VH chain of said human anti-human IL-6 antibody bound to a gene fragment coding for a VL chain of said human anti-human IL-6 antibody;
wherein complementarity determining regions (CDR1 to CDR3) of said VH chain have the following amino acid sequences:
CDR1:
Lys Tyr Tyr Met Ala
(SEQ ID NO: 5)
CDR2:
Thr Ile Ser Asn Ser Gly Asp Ile Ile
(SEQ ID NO: 6)
Asp Tyr Ala Asp Ser Val Arg Gly
CDR3:
Glu Tyr Phe Phe Ser Phe Asp Val
(SEQ ID NO: 7)
and/or complementarity determining regions (CDR1 to CDR3) of said VL chain have the following amino acid sequences:
CDR1:
Arg Ala Ser Gln Asp Ile Arg Asn
(SEQ ID NO: 8)
Trp Val Ala
CDR2:
Asp Gly Ser Ser Leu Gln Ser
(SEQ ID NO: 9)
CDR3:
Gln Gln Ser Asp Ser Thr Pro Ile
(SEQ ID NO: 10)
Thr Phe.
2. A gene fragment coding for a single chain Fv (hereinafter referred to as “scFv”) of a human anti-human IL-6 antibody that binds to human IL-6 with a dissactation constant (K D ) of 1.0×10 −8 M or less,
said gene fragment consisting of a gene fragment coding for a VH chain of said human anti-human IL-6 antibody bound to a gene fragment coding for a VL chain of said human anti-human IL-6 antibody,
wherein said VH chain has the amino acid sequence depicted in SEQ ID NO: 2 and/or said VL chain has the amino acid sequence depicted in SEQ ID NO: 4.